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Growth Hormone Production and Action in N1E-115 Neuroblastoma Cells

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Abstract

Neuroblastoma cells are undifferentiated cells derived from the neural crest and are commonly used as models for studying neural function. Mouse N1E-115 neuroblastoma cells are derived from cancerous tissue and provide a model for studying the oncogenesis of neural cells. As growth hormone (GH) has been implicated as an autocrine or paracrine involved in neural regulation and in the induction or progression of cancer, the possibility that N1E-115 cells are sites of GH production and GH action was assessed. Using RT-PCR, cultured N1E-115 cells were found to express the mouse GH and GH receptor (GHR) genes. Immunocytochemistry demonstrated that both of the translated proteins (GH and its receptor) were abundantly present in the cytoplasm of these cells and their co-localization was established by confocal cytochemistry. GH action in these cells was determined in cells cultured for 72 h in the presence or absence of 10–6 M or 10–9 M mouse GH, which induced neurite sprouting and increased axon growth. In summary, the expression of GH and its receptor in GH responsive tumor-derived N1E-115 neuroblastoma cells suggests they provide a useful experimental model to assess GH actions in neural function or neural oncogenesis.

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Acknowledgements

Supported by the Natural Science and Engineering Research Council (NSERC) of Canada and by studentships from NSERC and the Alberta Heritage Foundation for Medical Research to CG and BM.

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  1. Department of Physiology, University of Alberta, Edmonton, Alberta, T6G 2H7, Canada

    Chelsey Grimbly, Brent Martin, Edward Karpinski & Steve Harvey

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  1. Chelsey Grimbly
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Correspondence to Steve Harvey.

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Grimbly, C., Martin, B., Karpinski, E. et al. Growth Hormone Production and Action in N1E-115 Neuroblastoma Cells. J Mol Neurosci 39, 117–124 (2009). https://doi.org/10.1007/s12031-009-9194-7

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  • DOI: https://doi.org/10.1007/s12031-009-9194-7

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