Abstract
Synaptotagmin I is a key synaptic protein involved in both exocytosis and endocytosis. We aimed to investigate Synaptotagmin I expression in the anterior temporal neocortex of epilepsy patients, and to explore the possible role of Synaptotagmin I in refractory epilepsy. In the present study, 30 epilepsy patients were divided into refractory epilepsy and non-refractory epilepsy groups, another 15 histologically normal anterior temporal lobes from head trauma patients were used as control group. The results were compared among different groups. The findings were consistently observed using immunohistochemistry, immunofluorescence, and Western blotting technique. Synaptotagmin I was mainly expressed in the cytoplasm and cytomembrane of neurons. The expression of Synaptotagmin I in refractory epilepsy group was significantly higher than that in the control and non-refractory epilepsy groups. These findings provide new information in the epileptogenesis of refractory epilepsy, and suggest that Synaptotagmin I might be involved in human refractory epilepsy. Further studies will be required to elucidate the mechanism by which Synaptotagmin I plays role in refractory epilepsy.
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This material was based on work supported in part by the National Institutes of Health Grants 98-1-3457. We thank the patients and their families for their participation in this study. The authors sincerely thank Tiantan Hospital and Xuanwu Hospital of the Capital University of Medical Sciences, Xinqiao Hospital of the Third Military Medical University for the support in brain tissue procurement, and the local ethics committee and the National Board of the Medical Affairs for their support.
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Zheng Xiao and Yun Gong contributed equally to this work.
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Xiao, Z., Gong, Y., Wang, XF. et al. Altered Expression of Synaptotagmin I In Temporal Lobe Tissue of Patients With Refractory Epilepsy. J Mol Neurosci 38, 193–200 (2009). https://doi.org/10.1007/s12031-008-9143-x
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DOI: https://doi.org/10.1007/s12031-008-9143-x