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Downregulated Expression of E-cadherin and TP53 in Patients with Gastric Diseases: the Involvement of H. pylori Infection and Its Virulence Markers

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Abstract

Purpose

Gastritis caused by infection with Helicobacter pylori is characterized by chronic inflammation and damage in gastric tissue, which is a main risk factor for gastric cancer. Associated with H. pylori, the TP53 gene tumor suppressor and the cell adhesion glycoprotein epithelial cadherin develop a relevant role in the integrity and carcinogenesis of the epithelium. We aimed to detection of H. pylori and its main virulence markers and measured the messenger RNA (mRNA) expression levels of E-cadherin and TP53 genes.

Methods

The detection of H. pylori and its virulence markers, as well as the mRNA expression levels of E-cadherin and TP53 genes, were obtained for 161 samples of gastric biopsies including 37 with normal gastric tissue, 70 with gastritis, 24 from neoplastic tissue, and 27 from adjacent non-neoplastic by means of a quantitative real-time polymerase chain reaction.

Results

The mRNA expression levels of E-cadherin and TP53 were found to be decreased in patients with gastritis, independently of H. pylori infection. In samples from gastric patients, the neoplastic tissue showed an accentuated decrease of expression; on the other hand, the expression of E-cadherin was normal in adjacent non-neoplastic.

Conclusions

No evidence was found of the involvement of the cagA and vacA genes in the decreased expression of E-cadherin and TP53. The process of carcinogenesis is complex, and the decrease of the E-cadherin gene expression and TP53 gene expression appears to contribute significantly.

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Authors and Affiliations

  1. Pró-Reitoria de Pesquisa e Pós-Graduação, Universidade do Sagrado Coração, Rua Irmã Arminda, 10-50, Bauru, 17011160, Brazil

    Mariane Avante Ferraz, Luanna Munhoz Zabaglia, Weendelly Nayara Pereira, Wilson Aparecido Orcini, Spencer Luiz Marques Payão & Lucas T. Rasmussen

  2. Departamento de Genética e Biologia Molecular, Hemocentro, Faculdade de Medicina de Marília, Rua Monte Carmelo, 800, Marília, 17519050, Brazil

    Roger Willian de Labio & Agostinho Caleman Neto

  3. Disciplina de Genética, Departamento de Morfologia e Genética, Universidade Federal de São Paulo, Rua Botucatu, 740, São Paulo, 04023900, Brazil

    Fernanda Wisnieski, Danielle Queiroz Calcagno, Leonardo Caires Santos & Marilia de Arruda Cardoso Smith

  4. Núcleo de Pesquisa em Oncologia, Hospital João de Barros Barreto, Universidade Federal do Pará, Avenida Mundurucus, 4487, Belém, 66073000, Brazil

    Paulo Pimentel Assumpção

  5. Laboratório de Citogenética Humana, Instituto de Ciências Biológicas, Universidade Federal do Pará, Rua Augusto Correia, 01, Belém, 66075110, Brazil

    Rommel Rodriguez Burbano

  6. Departamento de Patologia, Universidade Federal de São Paulo, Rua Botucatu, 740, São Paulo, 04023000, Brazil

    Ricardo Artigiani

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  1. Mariane Avante Ferraz
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  2. Luanna Munhoz Zabaglia
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Correspondence to Lucas T. Rasmussen.

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The authors declare that there is no conflict of interests.

Funding Information

The authors would like to thank the São Paulo Research Foundation (FAPESP) for the funding of this research (grant numbers 20012/18333-3, 2013/21224-4, and 2013/21285-3).

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Ferraz, M.A., Zabaglia, L.M., Pereira, W.N. et al. Downregulated Expression of E-cadherin and TP53 in Patients with Gastric Diseases: the Involvement of H. pylori Infection and Its Virulence Markers. J Gastrointest Canc 47, 20–26 (2016). https://doi.org/10.1007/s12029-015-9782-4

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