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Protective effects of phytoestrogen α-zearalanol on beta amyloid25–35 induced oxidative damage in cultured rat hippocampal neurons

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Abstract

Although experimental evidence has shown that the neuroprotective effect from estrogen may benefit postmenopausal women, but the clinical use of estrogen was limited by the risk of increasing the cases of mammary and endometrial cancer. This study was designed to evaluate the neuroprotective effects of a novel phytoestrogen α-zearalanol (α-ZAL), on the cultured rat hippocampal neurons. Following a 24-h exposure of the cells to amyloid β-peptide fragment 25–35 (Aβ25–35), a significant reduction in cell survival and activities of total superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), as well as increased of malondialdehyde (MDA) were observed. Preincubation of the cells with α-ZAL or 17β-estradiol(17β-E2) prior to Aβ25–35 exposure elevated the cell survival and SOD and GSH-Px activities, and decreased the level of MDA. These data suggested that the phytoestrogen α-ZAL, like estrogen, may effectively antagonize Aβ25–35-induced cell toxicity, which might be beneficial for neurons.

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Acknowledgment

This work was supported by the grant from the Nation Basic Research Program of China (Project No.2007CB507400).

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Authors and Affiliations

  1. Department of Pharmacology, Institute of Basic Science, Chinese Academy of Medical Sciences & Peking Union Medical College, 5 Dong Dan San Tiao, Beijing, 100005, China

    Yi-Long Dong, Ping-Ping Zuo, Qing Li, Feng-Hua Liu & Shun-Ling Dai

  2. Peking Union Medical College Hospital, Beijing, 100730, China

    Qin-Sheng Ge

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  1. Yi-Long Dong
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  2. Ping-Ping Zuo
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  3. Qing Li
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Correspondence to Ping-Ping Zuo.

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Dong, YL., Zuo, PP., Li, Q. et al. Protective effects of phytoestrogen α-zearalanol on beta amyloid25–35 induced oxidative damage in cultured rat hippocampal neurons. Endocr 32, 206–211 (2007). https://doi.org/10.1007/s12020-007-9032-z

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  • DOI: https://doi.org/10.1007/s12020-007-9032-z

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