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Oct4 dependent chromatin activation is required for chicken primordial germ cell migration

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Abstract

Primordial germ cells (PGCs) are the undifferentiated progenitors of the gametes. Unlike the poor maintenance of cultured mammalian PGCs, the avian PGCs can be expanded in vitro indefinitely while preserving pluripotency and germline competence. In mammals, the Oct4 is the master transcription factor that ensures the stemness of pluripotent cells such as PGCs, but the specific function of Oct4 in chicken PGCs remains unclear. As expected, the loss of Oct4 in chicken PGCs reduced the expression of key pluripotency factors and promoted the genes involved in endoderm and ectoderm differentiation. Furthermore, the global active chromatin was reduced as shown by the depletion of the H3K27ac upon Oct4 suppression. Interestingly, the de-activated chromatin caused the down-regulation of adjacent genes which are mostly known regulators of cell junction, chemotaxis and cell migration. Consequently, the Oct4-deficient PGCs show impaired cell migration and could not colonize the gonads when re-introduced into the bloodstream of the embryo. We propose that, in addition to maintaining pluripotency, the Oct4 mediated chromatin activation is dictating chicken PGC migration.

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Data Availability

All data and materials during this study are available from the corresponding author on reasonable request.

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Acknowledgements

The authors wish to thank Jing Xu for technical support.

Funding

This work was supported by the National Natural Science Foundation of China (31872351) and National Key R&D Program of China (2021YFD1300103).

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Authors and Affiliations

  1. Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction of the Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, China

    Lu Meng, Sheng Wang, Haoyi Jiang, Yao Hua, Binxu Yin, Xiaochen Huang, Qiu Man, Heng Wang & Guiyu Zhu

  2. College of Animal Science and Technology, Shandong Agricultural University, Taian, China

    Guiyu Zhu

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  1. Lu Meng
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Contributions

Conceived and designed the experiments: LM and GZ. Performed the experiments: LM, HJ, BY, XH and QM. Analyzed the data: SW, YH, HW and LM. Wrote and reviewed the manuscript: LM, HW and GZ.

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Correspondence to Heng Wang or Guiyu Zhu.

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12015_2022_10371_MOESM1_ESM.pdf

Supplementary file1 (PDF 115 KB) Supplementary Figure 1. One major isoform of Oct4 was predominantly expressed in chicken PGCs and ESCs. (A) The RNAseq and qPCR data show that the Oct4A isoform was predominantly expressed in the chicken pluripotent PGCs and ESCs and the expression of Oct4B and Oct4B1 isoforms were extremely low and negligible. (B) The chicken Oct4 locus and the three isoforms detected in PGCs. Several candidate siRNAs were assayed for the knockdown efficiency and the most efficient siRNA was selected for subsequent experiments. The arrowhead points to the siRNA target region. The horizontal arrows indicate the primers.

Supplementary file2 (PDF 527 KB) Supplementary Figure 2. Correlation test between biological replicates of RNA-Seq.

Supplementary file3 (PDF 2815 KB) Supplementary Figure 3. Correlation test between biological replicates of ChIP-Seq.

Supplementary file4 (XLSX 9 KB) Supplementary Table 1

Supplementary file5 (XLSX 9 KB) Supplementary Table 2

Supplementary file6 (XLSX 10 KB) Supplementary Table 3

Supplementary file7 (XLSX 116 KB) Supplementary Table 4

Supplementary file8 (XLSX 18411 KB) Supplementary Table 5

Supplementary file9 (XLSX 1392 KB) Supplementary Table 6

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Meng, L., Wang, S., Jiang, H. et al. Oct4 dependent chromatin activation is required for chicken primordial germ cell migration. Stem Cell Rev and Rep 18, 2535–2546 (2022). https://doi.org/10.1007/s12015-022-10371-7

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