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Identification of a Novel High Yielding Source of Multipotent Adult Human Neural Crest-Derived Stem Cells

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Abstract

Due to their extraordinarily broad differentiation potential and persistence during adulthood, adult neural crest-derived stem cells (NCSCs) are highly promising candidates for clinical applications, particularly when facing the challenging treatment of neurodegenerative diseases or complex craniofacial injuries. Successful application of human NCSCs in regenerative medicine and pharmaceutical research mainly relies on the availability of sufficient amounts of tissue for cell isolation procedures. Facing this challenge, we here describe for the first time a novel population of NCSCs within the middle turbinate of the human nasal cavity. From a surgical point of view, high amounts of tissue are routinely and easily removed during nasal biopsies. Investigating the presence of putative stem cells in obtained middle turbinate tissue by immunohistochemistry, we observed Nestin+/p75NTR+/S100+/α smooth muscle actin (αSMA)− cells, which we successfully isolated and cultivated in vitro. Cultivated middle turbinate stem cells (MTSCs) kept their expression of neural crest and stemness markers Nestin, p75 NTR and S100 and showed the capability of sphere formation and clonal growth, indicating their stem cell character. Application of directed in vitro differentiation assays resulted in successful differentiation of MTSCs into osteogenic and neuronal cell types. Regarding the high amount of tissue obtained during surgery as well as their broad differentiation capability, MTSCs seem to be a highly promising novel neural crest stem cell population for applications in cell replacement therapy and pharmacological research.

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Authors and Affiliations

  1. Department of Otolaryngology, Head and Neck Surgery, Klinikum Bielefeld, Teutoburger Straße 50, 33604, Bielefeld, Germany

    Matthias Schürmann, Viktoria Brotzmann, Marlena Bütow & Holger Sudhoff

  2. Department of Cell Biology, University of Bielefeld, 33615, Bielefeld, Germany

    Johannes Greiner, Anna Höving, Christian Kaltschmidt & Barbara Kaltschmidt

  3. AG Molecular Neurobiology, University of Bielefeld, 33615, Bielefeld, Germany

    Barbara Kaltschmidt

Authors
  1. Matthias Schürmann
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  2. Viktoria Brotzmann
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  3. Marlena Bütow
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  4. Johannes Greiner
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  5. Anna Höving
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  6. Christian Kaltschmidt
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  7. Barbara Kaltschmidt
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  8. Holger Sudhoff
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Corresponding author

Correspondence to Holger Sudhoff.

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Conflicts of Interest

We have no conflicts of interest relevant to the content of this article. Human nasal middle turbinates and adipose tissue were obtained via routine nasal surgery after informed written consent according to local and international guidelines (Bezirksregierung Detmold/Münster). Isolation and further experimental procedures were ethically approved by the ethics commission of the Ärztekammer Westfalen-Lippe and the medical faculty of the Westfälische Wilhems-Universität (Münster, Germany).

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12015_2017_9797_MOESM1_ESM.jpg

Supplementary figure S1: Isolated hMSCs show characteristic morphology, expression profile and differentiation capability into mesodermal cell types. (A)Isolated MSCs show characteristic morphology. (B) RT-PCR and qPCR-analysis revealed the expression of MSC markers CD105, CD106, CD90, CD29, CD73 while lacking expression of non-MSC markers CD45 and CD13. (C) Expression of CD105 in isolated hMSCs was validated on protein level using flow cytometry. (D) hMSCs were able to differentiate into osteogenic cell types visualized by Alizarin red S-stained calcium deposition and adipogenic cells containing Oil red O-positive lipid droplets. (JPG 4956 KB)

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Schürmann, M., Brotzmann, V., Bütow, M. et al. Identification of a Novel High Yielding Source of Multipotent Adult Human Neural Crest-Derived Stem Cells. Stem Cell Rev and Rep 14, 277–285 (2018). https://doi.org/10.1007/s12015-017-9797-2

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  • DOI: https://doi.org/10.1007/s12015-017-9797-2

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