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Prediction of Ubiquitin Ligase Nrdp1-Associated Proteins in Glioma Database

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Abstract

The ubiquitin proteasome pathway is conserved from yeast to mammals and is necessary for the targeted degradation of most short-lived proteins in eukaryotic cells. Its protein substrates include cell cycle regulatory proteins and proteins that are not properly folded in the endoplasmic reticulum. Owing to the ubiquity of its protein substrates, ubiquitination regulates a variety of cellular activities, including cell proliferation, apoptosis, autophagy, endocytosis, DNA damage repair, and immune response. With new genomic data continuously being obtained, ubiquitination through genomic data analysis will be an effective method. We obtained 83 overlapping genes from four glioma databases, which differed from ubiquitin ligase Nrdp1 expression, including 36 downregulated and 47 upregulated genes. The KEGG pathways, molecular functions, cellular components, and biological processes potentially associated with Nrdp1 were obtained using GSEA and Cytoscape. In human gliomas, differences in the expression of Nrdp1 were identified between nontumor brain tissue and different glioma tissues, but no difference in expression was found between low‑grade glioma (LGG) and anaplastic glioma (AG). In survival analysis, we found no significant association between Nrdp1 expression level and patient prognosis.

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Funding

The present study was supported by the Foundation of Jiangsu Provincial Health Department (grant no. YG201514), Xuzhou Medical University (grant no. 2018KJ09).

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Author notes

  1. These authors contributed equally: Yong Liu, Mingwei Jin, Yong Gao, Yuan Wang

Authors and Affiliations

  1. Department of Neurosurgery, Brain Hospital, Affiliated Hospital of Xuzhou Medical University, Xuzhou, 221002, Jiangsu, PR China

    Yong Liu & Lei Wang

  2. Department of Hematology, Xuzhou Children’s Hospital of Xuzhou Medical University, Xuzhou, 221006, Jiangsu, PR China

    Mingwei Jin, Yuan Wang & Chengmin Xuan

  3. Department of Orthopaedics, Xuzhou Children’s Hospital of Xuzhou Medical University, Xuzhou, 221006, Jiangsu, PR China

    Yong Gao

  4. Department of Clinical Medicine, Nanjing Medical University, Nanjing, 211166, Jiangsu, PR China

    Shengbai Xue

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  1. Yong Liu
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  4. Yuan Wang
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  6. Lei Wang
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  7. Chengmin Xuan
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Correspondence to Chengmin Xuan.

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Liu, Y., Jin, M., Gao, Y. et al. Prediction of Ubiquitin Ligase Nrdp1-Associated Proteins in Glioma Database. Cell Biochem Biophys 78, 301–308 (2020). https://doi.org/10.1007/s12013-020-00926-1

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  • DOI: https://doi.org/10.1007/s12013-020-00926-1

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