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The Study of the Effect of Hedyotis diffusa on the Proliferation and the Apoptosis of the Cervical Tumor in Nude Mouse Model

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Abstract

To study the inhibitory effect of Hedyotis diffusa on cervical cancer and its underlining biomolecular mechanism. Human cervical carcinoma nude mice xenograft was established and the mice were treated by intra-gastric administration of boiled and concentrated Hedyotis diffusa. When the tumor grew to 10 mm in diameter, the mice were randomly divided into Hedyotis diffusa Willd. (HDW) group and control group. The tumor inhibitory rate, survival time, and the expression rate of Ki-67 protein in Hela cells as well as tumor cell apoptosis were compared between these two groups. Hedyotis diffusa had inhibitory effect on cervical cancer cells and induced apoptosis of Hela cells. The expression of Ki-67 protein significantly decreased (P < 0.05) in HDW group, and the mean survival time of the mice was significantly extended (P < 0.05). Hedyotis diffusa directly inhibited the proliferation of cervical cancer cells and induced apoptosis of the tumor cells. It has a positive effect for the treatment of cervical cancer to achieve the goal of clearing the heat, removing the toxins, eliminating the stasis, and dissolving the masses.

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    Authors and Affiliations

    1. Department of Traditional Chinese Medicine, Central Hospital of Xuzhou, Xuzhou, 221000, China

      Peiying Zhang

    2. Department of Gynecology, Central Hospital of Xuzhou, Xuzhou, 221000, China

      Bei Zhang, Juan Gu & Fangfang Hu

    3. Department of Urology, The Affiliated School of Clinical Medicine of Xuzhou Medical College, Xuzhou Central Hospital, 199 Jie Fang Nan Road, Xuzhou, 221009, People’s Republic of China

      Lin Hao & Conghui Han

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    1. Peiying Zhang
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    2. Bei Zhang
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    3. Juan Gu
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    4. Lin Hao
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    5. Fangfang Hu
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    6. Conghui Han
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    Correspondence to Conghui Han.

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    Peiying Zhang and Bei Zhang contributed equally to this work as co-first authors.

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    Zhang, P., Zhang, B., Gu, J. et al. The Study of the Effect of Hedyotis diffusa on the Proliferation and the Apoptosis of the Cervical Tumor in Nude Mouse Model. Cell Biochem Biophys 72, 783–789 (2015). https://doi.org/10.1007/s12013-015-0532-9

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    • DOI: https://doi.org/10.1007/s12013-015-0532-9

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