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RETRACTED ARTICLE: miR-200 Regulates Epithelial–Mesenchymal Transition in Anaplastic Thyroid Cancer via EGF/EGFR Signaling

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This article was retracted on 30 July 2019

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Abstract

This study was set to study the molecular mechanism underlying how miR-200 regulates EGF/EGFR signaling to involve in epithelial–mesenchymal transition (EMT) in anaplastic thyroid cancer (ATC) cells. Loss-of-function experiments of EGFR silencing by siRNA transfection was performed. Transfection of pre-miR-200s or anti-miR-200s was used to increase or decrease miR-200 transcripts. Real-time PCR, Western blot, immunohistochemistry, and transwell experiments were performed to determine the role of miR-200s in EMT and its role in EGF/EGFR-mediated EMT in vitro and in vivo. EGF/EGFR signaling activation increased the expression of mesenchymal marker vimentin in Nthy-ori 3-1 cells and decreased the expression of endothelial maker E-cadherin. EGF stimulation led to increased RhoA expression in Nthy-ori 3-1 cells. EGFR silencing resulted in decreased RhoA expression in SW1736 and ARO cells. EGF stimulation led to down-regulation of miR-200s and EMT. Restoration of miR-200 expression by pre-miR-200a/c transfection reversed the process, including increased E-cadherin and decreased vimentin. Down-regulation of miR-200 by anti-miR-200 effectively reduced miR-200. Matrigel invasion assay proved that restoration of miR-200 expression counteracted invasiveness. EGFR silencing decreased invasiveness in SW1736 cells, while down-regulation of miR-200s restored invasiveness. Xenograft tumors of SW1736 cells with cotransfection of anti-miR-200s and EGFR siRNA which kept the similar E-cadherin and vimentin expression with the untransfected controls. In ATC cells, miR-200s play a central role in EGF/EGFR-mediated invasiveness in vitro and EMT in vivo.

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  • 30 July 2019

    An amendment to this paper has been published and can be accessed via a link at the top of the paper.

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Authors and Affiliations

  1. Department of Endocrinology, Henan University Huaihe Hospital, Kaifeng, 475000, China

    Lei Xue, Dongyue Su, Dan Li, Wei Gao, Rongrong Yuan & Wuyan Pang

Authors
  1. Lei Xue
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  2. Dongyue Su
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  3. Dan Li
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  4. Wei Gao
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  5. Rongrong Yuan
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  6. Wuyan Pang
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Corresponding author

Correspondence to Wuyan Pang.

Additional information

Lei XUE and Dongyue SU have contributed equally.

The Editor-in-Chief has retracted this article due to significant overlap with a previously published article by a different author group. The authors have not responded to any correspondence regarding this retraction.

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Xue, L., Su, D., Li, D. et al. RETRACTED ARTICLE: miR-200 Regulates Epithelial–Mesenchymal Transition in Anaplastic Thyroid Cancer via EGF/EGFR Signaling. Cell Biochem Biophys 72, 185–190 (2015). https://doi.org/10.1007/s12013-014-0435-1

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  • DOI: https://doi.org/10.1007/s12013-014-0435-1

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