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Effects of Recombinant Human AMH on SCF Expression in Human Granulosa Cells

  • Translational Biomedical Research
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Abstract

The aim of the present study was to investigate the effects of recombinant human anti-mullerian hormone (rhAMH) on Stem Cell Factor (SCF) expression in human granulosa cells (GCs). GCs were obtained from infertile patients undergoing IVF-ET cycles and cultured with 20 ng/ml of rhAMH. The levels of SCF mRNA and protein were detected in both matched and experimental group by real-time PCR, immunofluorescence, and ELISA, respectively, on day 4 of culture. We found that human GCs expressed SCF mRNA and protein, and SCF expression in the experimental group was significantly lower than that in the matched group (p < 0.05). We further showed that rhAMH inhibited SCF expression at mRNA and protein levels.

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Acknowledgments

This work was supported by Grants from National Natural Science Foundation of China (No. 81260109); Natural Science Foundation of Ningxia Province, China (No. NZ10115); and State Key Foundation of Science and Technology of Ningxia, China (No. 2010297).

Conflict of interest

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the article.

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Authors and Affiliations

  1. The Center for Reproductive Medicine, First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, Henan, China

    Rong Hu & Ying-Pu Sun

  2. Reproductive Medicine Center, National Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education in Ningxia, General Hospital of Ningxia Medical University, Yinchuan, 750004, China

    Rong Hu, Yan Lou, Fei-Miao Wang, Hui-Ming Ma, Xing Wu & Juan Li

  3. Reproductive Medicine Center, Department of Obstetrics and Gynecology, Northern Jiangsu People’s Hospital, Yangzhou University, Yangzhou, 225001, China

    Xiao-Mei Zhang

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  1. Rong Hu
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  2. Yan Lou
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  3. Fei-Miao Wang
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  4. Hui-Ming Ma
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  5. Xing Wu
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  6. Xiao-Mei Zhang
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  7. Juan Li
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  8. Ying-Pu Sun
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Correspondence to Ying-Pu Sun.

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Hu, R., Lou, Y., Wang, FM. et al. Effects of Recombinant Human AMH on SCF Expression in Human Granulosa Cells. Cell Biochem Biophys 67, 1481–1485 (2013). https://doi.org/10.1007/s12013-013-9649-x

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  • DOI: https://doi.org/10.1007/s12013-013-9649-x

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