Abstract
The present report studied potential association of the rs1800925(C/T) single nucleotide polymorphism (SNP) of the Interleukin (IL)-13 gene promoter with idiopathic pulmonary fibrosis (IPF) in patients of Chinese Han ethnicity. Seventy patients with IPF were enrolled and divided into three subgroups: group A (61–79 % pred. DLCO; n = 22), group B (51–60 % pred. DLCO; n = 20), and group C (≤50 % pred. DLCO; n = 28). Control group consisted of 80 healthy individuals of Chinese Han ethnicity. The SNP rs1800925(C/T) was genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR–RFLP) analysis. The IL-13 CC genotype was present in 28/70 (40.0 %), homozygous TT in 6/70 (8.6 %) and heterozygous CT in 36/70 (51.4 %) patients with IPF. In control group, these genotypes were present in 30/80 (37.5 %), 11/80 (13.75 %), 39/80 (48.75 %), respectively, indicating that the distribution of the above three genotypes was not significantly different between patients with IPF and healthy controls. When the patients were stratified according to their DLCO and DLCO/VA, the frequencies of genotypes CT and TT in the groups A, B, and C were, respectively, 40.9 % (9/22), 50 % (10/20), and 82.1 % (23/28). Thus, significant differences in the distribution of alleles at −1112 region of IL-13 gene were observed among the study groups A, B, and C, with the highest frequency in group C (p < 0.05). In conclusion, the rs1800925 T allele of the IL-13 gene is associated with worse pulmonary function in patients with IPF of Chinese Han ethnicity.
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de Waal Malefyt, R., Figdor, C. G., Huijbens, R., Mohan-Peterson, S., Bennett, B., Culpepper, J., et al. (1993). Effects of IL-13 on phenotype, cytokine production, and cytotoxic function of human monocytes. Comparison with IL-4 and modulation by IFN-gamma or IL-10. Journal of Immunology, 151, 6370–6381.
Zurawski, G., & de Vries, J. E. (1994). Interleukin 13, an interleukin 4-like cytokine that acts on monocytes and B cells, but not on T cells. Immunology Today, 15, 19–26.
Wills-Karp, M. (2000). The gene encoding interleukin-13: a susceptibility locus for asthma and related traits. Respiratory Research, 1, 19–23.
Chen, Q., Rabach, L., Noble, P., Zheng, T., Lee, C. G., Homer, R. J., et al. (2005). IL-11 receptor alpha in the pathogenesis of IL-13-induced inflammation and remodeling. Journal of Immunology, 174, 2305–2313.
Elias, J. A., Zheng, T., Lee, C. G., Homer, R. J., Chen, Q., Ma, B., et al. (2003). Transgenic modeling of interleukin-13 in the lung. Chest, 123, 339S–345S.
Heinzmann, A., Mao, X. Q., Akaiwa, M., Kreomer, R. T., Gao, P. S., Ohshima, K., et al. (2000). Genetic variants of IL-13 signalling and human asthma and atopy. Human Molecular Genetics, 9, 549–559.
Jakubzick, C., Choi, E. S., Kunkel, S. L., Evanoff, H., Martinez, F. J., Puri, R. K., et al. (2004). Augmented pulmonary IL-4 and IL-13 receptor subunit expression in idiopathic interstitial pneumonia. Journal of Clinical Pathology, 57, 477–486.
Jakubzick, C., Choi, E. S., Joshi, B. H., Keane, M. P., Kunkel, S. L., Puri, R. K., et al. (2003). Therapeutic attenuation of pulmonary fibrosis via targeting of IL-4- and IL-13-responsive cells. Journal of Immunology, 171, 2684–2693.
Steele, M. P., Speer, M. C., Loyd, J. E., Brown, K. K., Herron, A., Slifer, S. H., et al. (2005). Clinical and pathologic features of familial interstitial pneumonia. American Journal of Respiratory and Critical Care Medicine, 172, 1146–1152.
Radhu, G., & Hert, R. (1993). Interstitial lung diseases: Genetic predisposition and inherited interstitial lung diseases. Seminars in Respiratory Medicine, 14, 323–332.
Howard, T. D., Whittaker, P. A., Zaiman, A. L., Koppelman, G. H., Xu, J., Hanley, M. T., et al. (2001). Identification and association of polymorphisms in the interleukin-13 gene with asthma and atopy in a Dutch population. American Journal of Respiratory Cell and Molecular Biology, 25, 377–384.
van der Pouw Kraan, T. C., Kucukaycan, M., Bakker, A. M., Baggen, J. M., van der Zee, J. S., Dentener, M. A., et al. (2002). Chronic obstructive pulmonary disease is associated with the −1055 IL-13 promoter polymorphism. Genes and Immunity, 2002(3), 436–439.
American Thoracic Society. (2000). Idiopathic pulmonary fibrosis: Diagnosis and treatment. International consensus statement. American Thoracic Society (ATS), and the European Respiratory Society (ERS). American Journal of Respiratory Cell and Molecular Biology, 161, 646–664.
Cherniack, R. M., Colby, T. V., Flint, A., Thurlbeck, W. M., Waldron, J. A, Jr, Ackerson, L., et al. (1995). Correlation of structure and function in idiopathic pulmonary fibrosis. American Journal of Respiratory and Critical Care Medicine, 151, 1180–1188.
Staples, C. A., Muller, N. L., Vedal, S., Abboud, R., Ostrow, D., & Miller, R. R. (1987). Usual interstitial pneumonia: correlation of CT with clinical, functional, and radiologic findings. Radiology, 162, 377–381.
Wells, A. U., King, A. D., Rubens, M. B., Cramer, D., du Bois, R. M., & Hansell, D. M. (1997). Lone cryptogenic fibrosing alveolitis: a functional-morphologic correlation based on extent of disease on thin-section computed tomography. American Journal of Respiratory and Critical Care Medicine, 155, 1367–1375.
Howard, T. D., Koppelman, G. H., Xu, J., Zheng, S. L., Postma, D. S., Meyers, D. A., et al. (2002). Gene-gene interaction in asthma: IL4RA and IL13 in a Dutch population with asthma. American Journal of Human Genetics, 70, 230–236.
Fujimoto, K., Kubo, K., Yamaguchi, S., Honda, T., & Matsuzawa, Y. (1995). Eosinophil activation in patients with pulmonary fibrosis. Chest, 108, 48–54.
Taniguchi, H., Katoh, S., Kadota, J., Matsubara, Y., Fukushima, K., Mukae, H., et al. (2000). Interleukin 5 and granulocyte-macrophage colony-stimulating factor levels in bronchoalveolar lavage fluid in interstitial lung disease. European Respiratory Journal, 16, 959–964.
Graves, P. E., Kabesch, M., Halonen, M., Holberg, C. J., Baldini, M., Fritzsch, C., et al. (2000). A cluster of seven tightly linked polymorphisms in the IL-13 gene is associated with total serum IgE levels in three populations of white children. The Journal of Allergy and Clinical Immunology, 105, 506–513.
Wen, H., Kang, J., & Li, Z. (2004). Correlation between eosinophil and pulmonary function in idiopathic pulmonary fibrosis patients. Chinese Journal of Tuberculosis and Respiratory Diseases, 27, 698–699. (in Chinese).
Pincus, S. H., Ramesh, K. S., & Wyler, D. J. (1987). Eosinophils stimulate fibroblast DNA synthesis. Blood, 70, 572–574.
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Ming Ding and Huaming Sheng contributed equally to the study and should be considered co-first authors.
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Ding, M., Sheng, H., Shen, W. et al. Association of the SNP rs1800925(C/T) in the Interleukin-13 Gene Promoter with Pulmonary Function in Chinese Han Patients with Idiopathic Pulmonary Fibrosis. Cell Biochem Biophys 67, 905–909 (2013). https://doi.org/10.1007/s12013-013-9580-1
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DOI: https://doi.org/10.1007/s12013-013-9580-1