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The Association of the Metalloproteinase-3 Gene Promoter Polymorphisms and the Middle Cerebral Artery Stenosis

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Abstract

To investigate the association of single nucleotide polymorphism in the matrix metalloproteinase-3 (MMP3) gene promoter with the susceptibility to the middle cerebral artery stenosis. A case–control study was performed by determining the genotype of MMP3 gene promoter region using polymerase chain reaction-restriction fragment length polymorphism in 119 patients with middle cerebral artery stenosis documented by transcranial Doppler compared to 92 control patients. The frequencies of 5A and 6A alleles in MMP3 promoter region were 16.0 and 84.0% respectively in case group compared to 15.8 and 84.2% in control group with no significant difference between the two groups (P > 0.05). No significant difference was also observed in the distribution of genotypes 5A/5A,5A/6A, and 6A/6A between middle cerebral artery stenosis and control groups. Compared to 5A/5A + 5A/6A genotypes,the 6A/6A genotype did not significantly modify the risk of developing the middle cerebral artery stenosis. The MMP3–1171 dupA promoter polymorphisms are not valuable markers of susceptibility of the middle cerebral artery stenosis in this sample of population studied.

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References

  1. Gibbons, G. H., & Dzau, V. J. (1994). The emerging concept of vascular remodeling. New England Journal of Medicine, 330, 1431–1438.

    Article  PubMed  CAS  Google Scholar 

  2. Flex, A., Gaetani, E., Papaleo, P., Straface, G., Proia, A. S., Pecorini, G., et al. (2004). Proinflammatory genetic profiles in subjects with history of ischemic stroke. Stroke, 35, 2270–2275.

    Article  PubMed  CAS  Google Scholar 

  3. Galis, Z. S., & Khatri, J. J. (2002). Matrix metalloproteinases in vascular remodeling and atherogenesis: the good, the bad, and the ugly. Circulation Research, 90, 251–262.

    PubMed  CAS  Google Scholar 

  4. Zhou, X., Huang, J., Chen, J., Su, S., Chen, R., & Gu, D. (2004). Haplotype analysis of the matrix metalloproteinase 3 gene and myocardial infarction in a Chinese Han population. The Beijing atherosclerosis study. Thrombosis and Haemostasis, 92, 867–873.

    PubMed  CAS  Google Scholar 

  5. Ye, S., Eriksson, P., Hamsten, A., Kurkinen, M., Humphries, S. E., & Henney, A. M. (1996). Progression of coronary atherosclerosis is associated with a common genetic variant of the human stromelysin-1 promoter which results in reduced gene expression. The Journal of Biological Chemistry, 271, 13055–13060.

    Article  PubMed  CAS  Google Scholar 

  6. Ghilardi, G., Biondi, M. L., DeMonti, M., Turri, O., Guagnellini, E., & Scorza, R. (2002). Matrix metalloproteinase-1 and matrix metalloproteinase-3 gene promoter polymorphisms are associated with carotid artery stenosis. Stroke, 33, 2408–2412.

    Article  PubMed  CAS  Google Scholar 

  7. Chen J (2005) Medical Genetics. People’s Medical House, 2005. Beijing.

  8. Li, Y., Wang, Y., Kang, S., Zhang, J. H., Guo, W., Wang, N., et al. (2005). Association of single nucleotide polymorphism in matrix metalloproteinases promoter with susceptibility to ovarian cancer. Zhonghua Fu Chan Ke Za Zhi, 40, 472–475.

    PubMed  Google Scholar 

  9. Gao, B., & Li, Z. C. (2004). Association between serum matrix metalloproteinase-3 concentration and the promoter 5A/6A polymorphism in patients with coronary heart disease. Zhongguo Wei Zhong Bing Ji Jiu Yi Xue, 16, 536–539.

    PubMed  CAS  Google Scholar 

  10. Brenner, D., Labreuche, J., Touboul, P. J., Schmidt-Petersen, K., Poirier, O., Perret, C., et al. (2006). Cytokine polymorphisms associated with carotid intima-media thickness in stroke patients. Stroke, 37, 1691–1696.

    Article  PubMed  CAS  Google Scholar 

  11. Henney, A. M., Wakeley, P. R., Davies, M. J., Foster, K., Hembry, R., Murphy, G., et al. (1991). Localization of stromelysin gene expression in atherosclerotic plaques by in situ hybridization. Proceedings of the National Academy of Sciences of the United States of America, 88, 8154–8158.

    Article  PubMed  CAS  Google Scholar 

  12. Gnasso, A., Motti, C., Irace, C., Carallo, C., Liberatoscioli, L., Bernardini, S., et al. (2000). Genetic variation in human stromelysin gene promoter and common carotid geometry in healthy male subjects. Arteriosclerosis, Thrombosis, and Vascular Biology, 20, 1600–1605.

    PubMed  CAS  Google Scholar 

  13. Rauramaa, R., Vaisanen, S. B., Luong, L. A., Schmidt-Trucksass, A., Penttila, I. M., Bouchard, C., et al. (2000). Stromelysin-1 and interleukin-6 gene promoter polymorphisms are determinants of asymptomatic carotid artery atherosclerosis. Arteriosclerosis, Thrombosis, and Vascular Biology, 20, 2657–2662.

    PubMed  CAS  Google Scholar 

  14. Rundek, T., Elkind, M. S., Pittman, J., Boden-Albala, B., Martin, S., Humphries, S. E., et al. (2002). Carotid intima-media thickness is associated with allelic variants of stromelysin-1, interleukin-6, and hepatic lipase genes: the Northern Manhattan Prospective Cohort Study. Stroke, 33, 1420–1423.

    Article  PubMed  Google Scholar 

  15. Irace, C., Cortese, C., Migale, M., Liberatoscioli, L., Mannucci, L., Federici, G., et al. (2008). Stromelysin gene promoter polymorphism and common carotid geometry in diabetic subjects. International Angiology, 27, 413–418.

    PubMed  CAS  Google Scholar 

  16. Lin, W., & Wu, J. (2008). The correlation study of metalloproteinase-3 gene polymorphism and ischemic stroke. Eleventh National Neurology compilation of academic Changchun, 8–1, 654.

    Google Scholar 

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Acknowledgments

The authors thank Dr. Yingqi Xing from the First Hospital of Jilin University and Dr. Guirong Zhang from the Biochemical Laboratory of Jilin University for their helpful advice and assistance in this study. The support of colleagues and staff from the Neurology Department of the First Hospital of Jilin University is gratefully acknowledged.

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Correspondence to Xiaonan Song.

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Fu, C., Xing, Y. & Song, X. The Association of the Metalloproteinase-3 Gene Promoter Polymorphisms and the Middle Cerebral Artery Stenosis. Cell Biochem Biophys 59, 185–189 (2011). https://doi.org/10.1007/s12013-010-9131-y

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