Abstract
Arsenic is a toxic metal, which ultimately leads to cell apoptosis. TLR4 signaling pathway played a key role in immunomodulatory. Therefore, alterations in related proteins on the TLR4 signaling pathway induced by arsenic exposure was systematically reviewed and analyzed by meta-analysis. Some databases were searched including PubMed, Web of Science, China National Knowledge Infrastructure (CNKI), and WANFANG MED ONLINE. The results of NF-κB, IKK, NF-κBp65, phospho-NF-κBp65, and TLR4 expressions were analyzed by Review Manage 5.3. In the arsenic intervention group, NF-κB, phospho-NF-κBp65, and TLR4 expression levels were higher than the control group, respectively. SMD and 95%CI were 11.29 (6.34, 16.24), 4.71(1.73, 7.68), and 5.79 (-4.22, 15.80). Compared to controls, in the exposed group, IKK levels were found to be 38.11-fold higher (Z = 0.97; P = 0.33); NF-κBp65 levels were found to be 0.92-fold higher (Z = 3.33; P = 0.0009) for normal cells and tissue, while IKK levels were found to be 5.18-fold lower (Z = 5.34; P < 0.0001); NF-κBp65 levels were found to be 2.01-fold lower (Z = 3.87; P = 0.0001) for abnormal cells. With comparing of low dose, high dose of arsenic exposure was found to reduce the expression of NF-κB, but increase the expression of NF-κBp65. This review supports the alterations in related proteins on the TLR4 signaling pathway induced by arsenic exposure, which is helpful to provide theoretical basis for the mechanism of toxicity of arsenic-induced immune system damage.
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This work was supported by Research Project Supported by Shanxi Scholarship Council of China (grant number 2021–085); Start-up Foundation for Doctors of Shanxi Medical University (grant number 03201523); National nature science foundation of China (grant number 82173644), and Natural Science Foundation of Shanxi [grant number 202103021224242].
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All authors contributed to the study conception and design. Material preparation, data collection, and analysis were performed by Nanxin Ma and Xiaolong WU. The first draft of the manuscript was written by Nanxin Ma and Jian Guo, and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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Ma, N., Guo, J., Wu, X. et al. Meta-analysis of TLR4 pathway-related protein alterations induced by arsenic exposure. Biol Trace Elem Res 201, 3290–3299 (2023). https://doi.org/10.1007/s12011-022-03426-w
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DOI: https://doi.org/10.1007/s12011-022-03426-w