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Brain Boron Level, DNA Content, and Myeloperoxidase Activity of Metformin-Treated Rats in Diabetes and Prostate Cancer Model

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Abstract

In this study, the effect of metformin on boron levels and oxidative brain damage in rats due to diabetes and prostate cancer was investigated for the first time. Myeloperoxidase (MPO) activity and the amount of DNA were investigated as tissue oxidative and toxic damage parameters. In Copenhagen rats, Dunning prostate cancer was induced using high metastatic MAT-Lylu cells and diabetes was induced by single dose of streptozotocin (STZ) injection. Metformin was administered for 14 days after diabetes and prostate cancer induced. The rats were divided into six groups as follows: control group, diabetic group (D), cancer group (C), diabetic + cancer (DC) group, cancer + metformin (CM) group, diabetic + cancer + metformin (DCM) group. At the end of the experiment, brains were removed. Significant decrease of brain boron levels and significant elevation of MPO activity and DNA levels were observed in D, C, and DC groups as compared to control group. The effect of diabetes induction on the brain boron levels was much more than prostate cancer induction. The administration of metformin with CM and DCM obviously declined MPO activity and increased brain boron levels almost near to control group level. In conclusion, this study shows that the protective effect of metformin against brain damage in STZ-induced diabetic rats with Dunning prostate cancer may also be related to increased boron levels. The boron levels may be a novel indicator of reduced toxic and oxidative stress. Furthermore, the distribution and mechanism of action of boron should be clarified.

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Authors and Affiliations

  1. Department of Basic Medical Sciences, Biochemistry, Faculty of Dentistry, Marmara University, Basibuyuk Road 9/3 34854 Basibuyuk, Maltepe, Istanbul, Turkey

    Armagan Begum Ozel & Aysen Yarat

  2. Department of Chemistry, Faculty of Engineering, Istanbul University- Cerrahpasa, Istanbul, Turkey

    Eda Dagsuyu & Refiye Yanardag

  3. Department of Histology and Embryology, Faculty of Medicine, Halic University, Istanbul, Turkey

    Pınar Koroglu Aydın

  4. Department of Biology, Faculty of Science, Istanbul University, Istanbul, Turkey

    Ilknur Bugan & Omur Karabulut Bulan

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  1. Armagan Begum Ozel
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  2. Eda Dagsuyu
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  4. Ilknur Bugan
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  5. Omur Karabulut Bulan
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Contributions

Armagan Begüm Özel: investigation, methodology, resources, writing-original draft, writing-review and editing, visualization. Eda Dagsuyu: methodology, resources, writing-review and editing, visualization. Pınar Koroglu Aydın: methodology, resources. Ilknur Bugan: methodology, resources. Omur Karabulut Bulan: methodology, resources, supervision. Refiye Yanardag: conceptualization, methodology, resources, writing-review and editing, supervision. Aysen Yarat: conceptualization, methodology, formal analysis, resources, writing-original draft, writing-review and editing, supervision.

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Correspondence to Aysen Yarat.

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The experiments were carried out according to the guiding principles in the use of experimental animals and approved by the Istanbul University Animal Care and Use Committee (2014/28 - ethics committee decision number)

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Highlights

• The first study on the brain boron level in metformin treated rats in diabetes and prostate cancer model.

• Metformin’s protective effect against brain tissue may also be related to increasing boron levels.

• Increased brain boron level is an important factor to reduce oxidative stress.

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Ozel, A.B., Dagsuyu, E., Aydın, P.K. et al. Brain Boron Level, DNA Content, and Myeloperoxidase Activity of Metformin-Treated Rats in Diabetes and Prostate Cancer Model. Biol Trace Elem Res 200, 1164–1170 (2022). https://doi.org/10.1007/s12011-021-02708-z

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