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Protective Effect of Organic Selenium on Oxidative Damage and Inflammatory Reaction of Rabbit Kidney Induced by T-2 Toxin

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Abstract

T-2 toxin is a member of a class of mycotoxins produced by a variety of Fusarium species under appropriate temperature and humidity conditions and is a common contaminant in food and feedstuffs of cereal origin. Selenium is an indispensable element in animals, regulates a variety of biological functions of the body, and can antagonize metal and mycotoxin poisoning to a certain extent. However, the effect of selenium on kidney injury induced by T-2 toxin has not been reported. In this study, 50 New Zealand rabbits were divided into 5 groups (the control group, T-2 toxin group, low-dose Se + T-2 toxin group, medium-dose Se + T-2 toxin group, and high-dose Se + T-2 toxin group). Rabbits were examined after oral administration of different doses of selenomethionine (SeMet) for 21 days and after perfusion with 0.4 mg/kg T-2 toxin (or the same dose of olive oil in the control group) for 5 days. We found that T-2 toxin induced kidney function damage and increased the levels of ROS and the contents of inflammatory factors. Renal structure was pathologically damaged. However, we found that after pretreatment with 0.2 mg/kg SeMet, oxidative stress, the inflammatory response, and pathological damage induced by T-2 toxin were attenuated. The results indicate that a low dose (0.2 mg/kg) of SeMet effectively reversed T-2 toxin–induced kidney injury in rabbits.

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Data Availability

This published paper includes all data generated or analyzed during this work.

Abbreviations

WHO:

World Health Organization

ROS:

Reactive oxygen species

MDA:

Malondialdehyde

BBB:

Blood-brain barrier

GSH-Px:

Glutathione peroxidase

UREA:

Urea nitrogen

Crea:

Creatinine

H&E:

Hematoxylin & eosin

PAS:

Periodic acid-Schiff

IL-1β:

Interleukin-1β

TNF-α:

Tumor necrosis factor-α

SeMet:

Selenomethionine

References

  1. Escriva L, Font G, Manyes L (2015) In vivo toxicity studies of fusarium mycotoxins in the last decade: a review. Food Chem Toxicol 78:185–206. https://doi.org/10.1016/j.fct.2015.02.005

    Article  CAS  PubMed  Google Scholar 

  2. Organization WHJWHOTR (2002) Evaluation of certain food additives. Seventy-first report of the Joint FAO/WHO Expert Committee on Food Additives 891 (956):1–80

  3. Makowska K, Obremski K, Gonkowski S (2018) The impact of T-2 toxin on vasoactive intestinal polypeptide-like immunoreactive (VIP-LI) nerve structures in the wall of the porcine stomach and duodenum. Toxins (Basel) 10(4). https://doi.org/10.3390/toxins10040138

  4. Manish Adhikari AA-K, Negi B, Kaushik N, Kaushik NK, Adhikari A, Choi EH T-2 mycotoxin: toxicological effects and decontamination strategies. Oncotarget 8:33933–33952

  5. Wu J, Yang C, Liu J, Chen J, Huang C, Wang J, Liang Z, Wen L, Yi JE, Yuan Z (2019) Betulinic acid attenuates T-2-toxin-induced testis oxidative damage through regulation of the JAK2/STAT3 signaling pathway in mice. Biomolecules 9(12). https://doi.org/10.3390/biom9120787

  6. Adhikari M, Negi B, Kaushik N, Adhikari A, Al-Khedhairy AA, Kaushik NK, Choi EH (2017) T-2 mycotoxin: toxicological effects and decontamination strategies. Oncotarget 8(20):33933–33952. https://doi.org/10.18632/oncotarget.15422

    Article  PubMed  PubMed Central  Google Scholar 

  7. He SJ, Hou JF, Dai YY, Zhou ZL, Deng YF (2012) N-acetyl-cysteine protects chicken growth plate chondrocytes from T-2 toxin-induced oxidative stress. J Appl Toxicol 32(12):980–985. https://doi.org/10.1002/jat.1697

    Article  CAS  PubMed  Google Scholar 

  8. Ravindran J, Agrawal M, Gupta N, Rao PV (2011) Alteration of blood brain barrier permeability by T-2 toxin: role of MMP-9 and inflammatory cytokines. Toxicology 280(1–2):44–52. https://doi.org/10.1016/j.tox.2010.11.006

    Article  CAS  PubMed  Google Scholar 

  9. Shanu A, Groebler L, Kim HB, Wood S, Weekley CM, Aitken JB, Harris HH, Witting PK (2013) Selenium inhibits renal oxidation and inflammation but not acute kidney injury in an animal model of rhabdomyolysis. Antioxid Redox Signal 18(7):756–769. https://doi.org/10.1089/ars.2012.4591

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Dvorska JE, Pappas AC, Karadas F, Speake BK, Surai PF (2007) Protective effect of modified glucomannans and organic selenium against antioxidant depletion in the chicken liver due to T-2 toxin-contaminated feed consumption. Comp Biochem Physiol C Toxicol Pharmacol 145(4):582–587. https://doi.org/10.1016/j.cbpc.2007.02.005

    Article  CAS  PubMed  Google Scholar 

  11. Guerre P, Eeckhoutte C, Burgat V, Galtier P (2000) The effects of T-2 toxin exposure on liver drug metabolizing enzymes in rabbit. Food Addit Contam 17(12):1019–1026. https://doi.org/10.1080/02652030050207819

    Article  CAS  PubMed  Google Scholar 

  12. Qu J, Wang W, Zhang Q, Li S (2020) Inhibition of lipopolysaccharide-induced inflammation of chicken liver tissue by selenomethionine via TLR4-NF-kappa B-NLRP3 signaling pathway. Biol Trace Elem Res 195(1):205–214. https://doi.org/10.1007/s12011-019-01841-0

    Article  CAS  PubMed  Google Scholar 

  13. Chan PK, Gentry PAJT, Pharmacology A (1984) LD50 values and serum biochemical changes induced by. T-2 toxin in rats and rabbits 73(3):402–410

    CAS  Google Scholar 

  14. Glávits R, Ványi A, Fekete S, Tamás JJAVH (1989) Acute toxicological experiment of. T-2 toxin in rabbits 37(1–2):75–79

    Google Scholar 

  15. Valko M, Leibfritz D, Moncol J, Cronin MTD, Mazur M, Telser J (2007) Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol 39(1):44–84

    Article  CAS  Google Scholar 

  16. Doi K, Uetsuka K (2011) Mechanisms of mycotoxin-induced neurotoxicity through oxidative stress-associated pathways. Int J Mol Sci 12(8):5213–5237. https://doi.org/10.3390/ijms12085213

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  17. Tian J, Yan J, Wang W, Zhong N, Tian L, Sun J, Min Z, Ma J, Lu SJ T-2 toxin enhances catabolic activity of hypertrophic chondrocytes through ROS-NF-kappa B-HIF-2 alpha pathway

  18. Wu Q-H, Wang X, Yang W, Nüssler AK, Xiong L-Y, Kuča K, Dohnal V, Zhang X-J, Yuan Z-H (2014) Oxidative stress-mediated cytotoxicity and metabolism of T-2 toxin and deoxynivalenol in animals and humans: an update. Arch Toxicol 88(7):1309–1326. https://doi.org/10.1007/s00204-014-1280-0

    Article  CAS  PubMed  Google Scholar 

  19. Wang N, Tan H-Y, Li S, Xu Y, Guo W, Feng Y (2017) Supplementation of micronutrient selenium in metabolic diseases: its role as an antioxidant. Oxidative Med Cell Longev. https://doi.org/10.1155/2017/7478523

  20. Brenneisen P, Steinbrenner H, Sies H Selenium, oxidative stress, and health aspects. 26 (4–5):0–267

  21. Lin RQ, Sun Y, Ye WC, Zheng T, Wen JK, Deng YQ (2019) T-2 toxin inhibits the production of mucin via activating the IRE1/XBP1 pathway. Toxicology 424:11. https://doi.org/10.1016/j.tox.2019.06.001

    Article  CAS  Google Scholar 

  22. Li Y, Zou N, Wang J, Wang KW, Li FY, Chen FX, Sun BY, Sun DJ (2017) TGF-beta1/Smad3 signaling pathway mediates T-2 toxin-induced decrease of type II collagen in cultured rat chondrocytes. Toxins (Basel) 9 (11). doi:https://doi.org/10.3390/toxins9110359

  23. Lin R, Sun Y, Ye W, Zheng T, Wen J, Deng Y (2019) T-2 toxin inhibits the production of mucin via activating the IRE1/XBP1 pathway. Toxicology 424:152230. https://doi.org/10.1016/j.tox.2019.06.001

    Article  CAS  PubMed  Google Scholar 

  24. Wang X, Liu Q, Ihsan A, Huang L, Dai M, Hao H, Cheng G, Liu Z, Wang Y, Yuan ZJ JAK/STAT Pathway plays a critical role in the proinflammatory gene expression and apoptosis of RAW264.7 cells induced by trichothecenes as DON and T-2 Toxin. 127 (2):412–424

  25. Ravindran J, Agrawal M, Gupta N, Rao PVLJT (2011) Alteration of blood brain barrier permeability by. T-2 toxin: Role of MMP-9 and inflammatory cytokines 280(1–2):44–52

    CAS  Google Scholar 

  26. Ott J, Promberger R, Kober F, Neuhold N, Tea M, Huber JC, Hermann MJ Hashimoto’s thyroiditis affects symptom load and quality of life unrelated to hypothyroidism: a prospective case–control study in women undergoing thyroidectomy for benign goiter. 21 (2):161–167

  27. Schrauzer GN (2003) The nutritional significance, metabolism and toxicology of selenomethionine. Adv Food Nutr Res 47:73–112. https://doi.org/10.1016/s1043-4526(03)47002-2

    Article  CAS  PubMed  Google Scholar 

  28. Patterson BH, Levander OA, Helzlsouer K, Mcadam PA, Lewis SA, Taylor PR, Veillon C, Zech LA (1989) Human selenite metabolism: a kinetic model. Am J Physiol 257(2):556–567

    Google Scholar 

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Acknowledgments

We thank the American Journal Experts (AJE) for its linguistic assistance during the preparation of this manuscript.

Funding

This work was supported by the Henan Provincial Key Research and Development and Promotion Project (192102110077 and 202102110093), the Key Research Project of Henan Province Colleges and Universities (19B230005), the Key Research Project of Henan Province Colleges and Universities (19B230005), and the Young Backbone Teachers Assistance Scheme of Henan Province Colleges and Universities (2019GGJS080).

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Author notes

  1. Yumei Liu and Ruiqi Dong contributed equally to this work.

Authors and Affiliations

  1. College of Animal Science and Technology, Henan University of Science and Technology, Kaiyuan Avenue 263, Luolong District, Luoyang City, 471023, Henan, People’s Republic of China

    Yumei Liu, Ruiqi Dong, Yuxiang Yang, Hui Xie, Yufeng Huang, Xiaoguang Chen & Ziqiang Zhang

  2. Medical College, Henan University of Science and Technology, Luoyang, 471023, Henan, People’s Republic of China

    Dongmei Wang

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  1. Yumei Liu
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Contributions

Yumei Liu, Ruiqi Dong, and Ziqiang Zhang conceived and designed the study. Yuxiang Yang, Hui Xie, Yufeng Huang, Xiaoguang Chen, and Dongmei Wang performed the experiments. This paper was written by Yumei Liu and Ruiqi Dong. The manuscript was reviewed and edited by Ziqiang Zhang. All authors have read and approved this manuscript.

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Correspondence to Ziqiang Zhang.

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The authors declare that they have no conflict of interest.

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All animal care and experimental protocols were conducted according to the University Policies on the Use and Care of Animals and were approved by the Institutional Animal Experiment Committee of Henan University of Science and Technology, China.

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Liu, Y., Dong, R., Yang, Y. et al. Protective Effect of Organic Selenium on Oxidative Damage and Inflammatory Reaction of Rabbit Kidney Induced by T-2 Toxin. Biol Trace Elem Res 199, 1833–1842 (2021). https://doi.org/10.1007/s12011-020-02279-5

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