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Boron Supplementation Promotes Osteogenesis of Tibia by Regulating the Bone Morphogenetic Protein-2 Expression in African Ostrich Chicks

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Abstract

The present study aimed to explore the effects of supplemental boron on osteogenesis of tibia and to investigate the possible relationship between additional boron and the expression of bone morphogenetic protein-2 (BMP-2) in tibia of ostrich chicks. Therefore, forty-eight African ostrich chicks (15 days old) were supplemented with 0 mg/L, 40 mg/L, 80 mg/L, 160 mg/L, 320 mg/L, and 640 mg/L of boron in drinking water for 75 days. The paraffin sections of tibia used to measure histomorphometric parameters by hematoxylin and eosin (HE) staining, Masson’s staining, and immunohistochemistry (IHC). Enzyme-linked immunosorbent assay was performed to assess the level of BMP-2, osteocalcin (BGP), glucocorticoids (GCs), osteoprotegerin (OPG), and receptor activator of nuclear factor kappa-B ligand (RANKL) in serum. TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling) technique was performed to detect the cell apoptosis. The results indicated that low dose of supplemental boron (40 mg/L–160 mg/L) in drinking water promotes bone development by increasing the mature ossein. The expression of BMP2 on 45 days was higher than 90 days. Serum level of BMP-2, BGP, and GCs changed significantly in groups with low dosage of boron, and OPG/RANKL ratio was upregulated from 0 to 160 mg/L. Cell apoptosis was least in 40 mg/L and 160 mg/L groups. Taken together, low dose of boron supplemented in drinking water could promote osteogenesis and growth and development of tibia by regulating the expression and secretion of BMP-2 and providing a dynamically balanced environment for tibia growth, development, and reconstruction by regulating the concentrations of BGP, GCs, and OPG/RANKL ratio in serum.

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Funding

This work was supported by the National Natural Science Foundation of China No. 31672504 and 31272517.

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Authors and Affiliations

  1. College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, People’s Republic of China

    Daiyun Zhu, Ke Xiao, Wei Wang, Lei Wang, Weiwei Qiu, Xinting Zheng, Hui Song, Huazhen Liu & Kemei Peng

  2. Section of Anatomy and Histology, Department of Basic Sciences, College of Veterinary and Animal Sciences (CVAS) Jhang, University of Veterinary and Animal Sciences (UVAS), Lahore, Pakistan

    Abdur Rahman Ansari

  3. College of Veterinary Medicine, Auburn University, Auburn, AL, 36849, USA

    Juming Zhong

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  1. Daiyun Zhu
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Contributions

Conceptualization, formal analysis, methodology, and writing (original draft): Daiyun Zhu

Writing and editing: Abdur Rahman Ansari

Conceptualization, performing experiments, formal analysis, and methodology: Xiao Ke, Wei Wang, and Lei Wang

Reviewing and editing: Weiwei Qiu and Xinting Zheng

Methodology and reviewing: Hui Song and Huazhen Liu

Conceptualization: Juming Zhong

Conceptualization, funding acquisition, methodology, writing, and editing: Kemei Peng

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Correspondence to Kemei Peng.

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Zhu, D., Ansari, A.R., Xiao, K. et al. Boron Supplementation Promotes Osteogenesis of Tibia by Regulating the Bone Morphogenetic Protein-2 Expression in African Ostrich Chicks. Biol Trace Elem Res 199, 1544–1555 (2021). https://doi.org/10.1007/s12011-020-02258-w

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