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Effects of Silver Nanoparticles on Burn Wound Healing in a Mouse Model

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Abstract

Healing of injuries caused by exposure to heat has been discussed in many studies, although a few drugs have been shown to produce satisfactory results. In this study, 100 healthy mice randomly allocated into four categories (each = 25 mice) were analyzed. A deep second-degree burn on the back of each mouse was created. The burns were dressed daily with either AgNPs or silver sulfadiazine over 28 days of treatment. Safety evaluation of the AgNP treatment was performed by measuring the deposition rate of silver in the liver, brain, and kidney of treated mice. In the murine burn model, the speed of wound healing and the antibacterial effect of AgNPs were better than those in the silver sulfadiazine group. Burn wounds treated with SSD appeared to display a greater degree of inflammation as notable by the three clinical signs of the inflammatory process such as redness and swelling which appeared to be less after wounds treated with AgNPs. Also, AgNP treatment modified leukocytic infiltration and reduced collagen degeneration in treated mice and enhanced healing processes that were confirmed by morphological and histological investigations. Beside the potential significant effects of AgNPs on reduction of some microorganism counts that routinely isolated from burn wounds included aerobic organisms as Staphylococcus aureus and Escherichia coli when compared to both SSD and control groups. The deposition kinetics of AgNPs revealed lower distribution in the liver, brain, and kidney than that in silver sulfadiazine-treated mice with respect to both SSD and control groups.

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Acknowledgments

The authors extend their appreciation to the Deanship of Scientific Research at King Saud University for funding this work through the research group project (RG-1438-066). The authors thank the Deanship of Scientific Research and RSSU at King Saud University for their technical support. Authors extend thanks to their respected institutes and universities.

Funding

The authors extend their appreciation to the Deanship of Scientific Research at King Saud University for funding this work through the research group project (RG-1438-066).

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Authors and Affiliations

  1. Department of Pharmacology, Faculty of Veterinary Medicine, Damanhour University, Damanhour, Egypt

    Lamiaa G. Wasef & Hazem M. Shaheen

  2. Department of Veterinary Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Damanhour University, Damanhour, Egypt

    Yasser S. El-Sayed & Dalia H. Samak

  3. Department of Medical Biophysics, Medical Research Institute, Alexandria University, Alexandria, Egypt

    Thanaa I. A. Shalaby

  4. Department of Poultry, Faculty of Agriculture, Zagazig University, Zagazig, 44511, Egypt

    Mohamed E. Abd El-Hack

  5. Department of Animal Production, College of Food and Agriculture Sciences, King Saud University, P.O. Box 2460, Riyadh, 11451, Saudi Arabia

    Abdullah Al-Owaimer, Islam M. Saadeldin, Hani Ba-Awadh & Ayman A. Swelum

  6. Department of Physiology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, 44511, Egypt

    Islam M. Saadeldin

  7. Department of Pharmacology, Faculty of Veterinary Medicine, Menoufia University, Menoufia, Egypt

    Amany El-mleeh

  8. Department of Theriogenology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, 44511, Egypt

    Ayman A. Swelum

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  1. Lamiaa G. Wasef
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Contributions

H.S. and Y.S. designed the study plan, collected literature, and drafted the manuscript. L.M. and A.A. helped in conducting research work. I.M.S., A.E., H.E., and A.S.A. provided technical help in the write-up of this manuscript. D.S. and M.E.A.E.-H. reviewed and performed the final check. All the authors read and approved the final manuscript.

Corresponding authors

Correspondence to Mohamed E. Abd El-Hack or Ayman A. Swelum.

Ethics declarations

The present study was performed in accordance with the recommendations of the Committee on the Ethics of Animal Experiments of Damanhour University, Egypt (DMU-2019-0023). All procedures and experiments followed the guidelines and were approved by the Local Ethic Commission of the Animal Experiments of Damanhour University with respect to animal experimentation and care of animals under study.

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Wasef, L.G., Shaheen, H.M., El-Sayed, Y.S. et al. Effects of Silver Nanoparticles on Burn Wound Healing in a Mouse Model. Biol Trace Elem Res 193, 456–465 (2020). https://doi.org/10.1007/s12011-019-01729-z

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