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Detection of Se, Vit. E, Vit. A, MDA, 8-OHdG, and CoQ10 Levels and Histopathological Changes in Heart Tissue in Sheep with White Muscle Disease

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Abstract

This study was carried out to determine vit. E, Se, vit. A, malondialdehyde (MDA), 8-hydroxy-2-deoxyguanosine (8-OHdG), and ubiquinone-10 (CoQ10) levels and histopathological changes in sheep with white muscle disease (WMD). A total of 30 sheep were used; 20 sheep with WMD were brought to our clinic for diagnosis and treatment at various times, and 10 healthy sheep were in the control group. The Se, vit. E, vit. A, MDA, 8-OHdG, and CoQ10 values of the healthy and WMD sheep were as follows: 0.917 ± 0.037, 0.790 ± 0.067; 1.190 ± 0.011, 1.090 ± 0.021; 5.400 ± 0.275, 5.200 ± 0.173; 1.602 ± 0.264, 2.636 ± 0.576; 0.656 ± 0.197, 1.485 ± 0.271; and 0.280 ± 0.044, 1.753 ± 0.551 respectively (p < 0.05). According to histopathological and immunohistochemical findings in the WMD group, hyaline degeneration, Zenker’s necrosis, and dystrophic calcification were observed in the muscle fibers. Immunohistochemically, 8-OHdG staining of the heart tissue determined a severe 8-OHdG expression in the WMD group. The findings of this study suggest that MDA, 8-OHdG, and CoQ10 values could be used as diagnostic and prognostic biomarkers in sheep affected with WMD.

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Authors and Affiliations

  1. Department of Pathology, Faculty of Veterinary Medicine, Ataturk University, Erzurum, Turkey

    Serkan Yildirim

  2. Department of Internal Diseases, Faculty of Veterinary Medicine, Van Yuzuncu Yil University, Van, Turkey

    Cumali Ozkan

  3. Department of Biochemistry, Faculty of Medicine, Van Yuzuncu Yil University, Van, Turkey

    Zubeyir Huyut

  4. Department of Physiology, Faculty of Veterinary Medicine, Ataturk University, Erzurum, Turkey

    Ali Çınar

Authors
  1. Serkan Yildirim
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  2. Cumali Ozkan
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  3. Zubeyir Huyut
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  4. Ali Çınar
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Correspondence to Ali Çınar.

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The experimental protocol was approved by the Unit Ethics Committee at Atatürk University (permit number: 2018/36).

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Yildirim, S., Ozkan, C., Huyut, Z. et al. Detection of Se, Vit. E, Vit. A, MDA, 8-OHdG, and CoQ10 Levels and Histopathological Changes in Heart Tissue in Sheep with White Muscle Disease. Biol Trace Elem Res 188, 419–423 (2019). https://doi.org/10.1007/s12011-018-1434-7

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  • DOI: https://doi.org/10.1007/s12011-018-1434-7

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