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Vinpocetine and Vasoactive Intestinal Peptide Attenuate Manganese-Induced Toxicity in NE-4C Cells

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An Erratum to this article was published on 14 June 2016

Abstract

Increased concentration of manganese (Mn) in the brain is known to be associated with excitotoxicity and neuroinflammation. Vinpocetine, an alkaloid derived from the plant Vinca minor L., basically shows its effect via phosphodiesterase inhibition and voltage-dependent Na+ channels. Vasoactive intestinal peptide (VIP) has gastrointestinal, vasomotor, muscular, and neuroprotective effects. The aim of this study was to examine the potential protective effects of vinpocetine and VIP against Mn toxicity in NE-4C neural stem cells (NSCs). VIP treatment at 1 μM and vinpocetine treatment at 2 μM concentrations were sufficient to yield maximum protection, and these concentrations were adopted in the following experiments. In this study, Mn treatment significantly increased lactate dehydrogenase (LDH) leakage, reactive oxygen species (ROS) production, and triggered cell death in NE-4C cultures. However, significant reduction in LDH release was observed following vinpocetine or VIP treatments when compared with control. Similar to these findings, vinpocetine or VIP treatments significantly reduced membrane degradation induced by Mn (p < 0.001). Moreover, vinpocetine attenuated Mn-induced decrease of mitochondrial membrane potential. Similarly, proapoptotic protein bax and ROS production significantly decreased in cells after incubation with vinpocetine (p = 0.01) or VIP in the presence of Mn (p < 0.001). Our study provides the evidence that both vinpocetine and VIP may exert protective effects via modulating oxidative stress and apoptosis in Mn-induced neurodegeneration in NE-4C cells.

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Acknowledgments

This study was supported by the Ege University Scientific Research Foundation (Project No: 13/TIP/003). The authors also acknowledge the Pharmaceutical Sciences Research Centre (FABAL) of Ege University, Faculty of Pharmacy.

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Authors and Affiliations

  1. Department of Physiology, School of Medicine, Faculty of Medicine, Ege University, 35100, Bornova, Izmir, Turkey

    Saylav Bora, Mumin Alper Erdogan & Taner Dagcı

  2. Department of Biochemistry, Faculty of Pharmacy, Ege University, 35100, Bornova, Izmir, Turkey

    Güliz Armagan & Elvin Sevgili

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  1. Saylav Bora
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  2. Mumin Alper Erdogan
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  3. Güliz Armagan
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Correspondence to Saylav Bora.

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An erratum to this article is available at http://dx.doi.org/10.1007/s12011-016-0775-3.

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Bora, S., Erdogan, M.A., Armagan, G. et al. Vinpocetine and Vasoactive Intestinal Peptide Attenuate Manganese-Induced Toxicity in NE-4C Cells. Biol Trace Elem Res 174, 410–418 (2016). https://doi.org/10.1007/s12011-016-0742-z

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  • DOI: https://doi.org/10.1007/s12011-016-0742-z

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