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Selenium Deficiency Attenuates Chicken Duodenal Mucosal Immunity via Activation of the NF-κb Signaling Pathway

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Abstract

Selenium (Se) deficiency can cause intestinal mucosal inflammation, which is related to activation of nuclear transcription factor kappa-B (NF-κB) signaling pathway. However, the mechanism of inflammatory response in chicken duodenal mucosa caused by Se deficiency and its relationship with the NF-κB signaling pathway remain elusive. In this study, we firstly obtained Se-deficient chickens bred with 0.01 mg/kg Se and the normal chickens bred with 0.4 mg/kg Se for 35 days. Then, NF-κB signaling pathway, secretory immunoglobulin A (SIgA), inflammatory cytokines, oxidized glutathione, glutathione peroxidase, and glutathione activities were determined. The results showed that Se deficiency obviously enhanced p50, p65, and p65 DNA-binding activities. The phosphorylation of IκB-α and phosphorylation of kappa-B kinase subunit alpha (IKKα) and IKKα were elevated, but IκB-α was decreased (P < 0.05). Moreover, Se deficiency reduced SIgA amount in the duodenal mucosa but increased the level of interleukin-1β (IL-1β), IL-17A, tumor necrosis factor-α (TNF-α), and interferon gamma (IFN-γ). In contrast, anti-inflammatory cytokines, such as TGF-β1 and IL-10, were significantly suppressed. Additionally, Se deficiency increased oxidized glutathione activity, whereas decreased glutathione peroxidase and glutathione activities (P < 0.05), suggesting that Se deficiency affected the regulation function of redox. Taken together, our results demonstrated that Se deficiency attenuated chicken duodenal mucosal immunity via activation of NF-κB signaling pathway regulated by redox activity, which suggested that Se is a crucial host factor involved in regulating inflammation.

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Acknowledgments

This work was supported by grants from the Postgraduate Research and Innovation Projects in Heilongjiang Province of China (YJSCX2014-203) and the Foundation of National Natural Science Project, P.R. China (No. 31272624 / C180802). We are grateful to Dr. Hua Zhang (College of Life Science and Technology, HeiLongJiang BaYi Agricultural University) for a critical reading of the manuscript. The authors wish to thank the study the participants and Shanghai Proteome Research Analysis Center of the Chinese Academy of Sciences for their contributions to this study. All of the authors have read the manuscript and have agreed to submit it, in its current form, for consideration for publication.

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Authors and Affiliations

  1. College of Life Sciences and Biotechnology, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang, 163319, People’s Republic of China

    Zhe Liu

  2. College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, 2 Xinyang Road, Daqing, Heilongjiang, 163319, People’s Republic of China

    Zhe Liu, Yanpeng Qu, Jianfa Wang & Rui Wu

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  1. Zhe Liu
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Correspondence to Rui Wu.

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Liu, Z., Qu, Y., Wang, J. et al. Selenium Deficiency Attenuates Chicken Duodenal Mucosal Immunity via Activation of the NF-κb Signaling Pathway. Biol Trace Elem Res 172, 465–473 (2016). https://doi.org/10.1007/s12011-015-0589-8

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