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Effect of Zinc on Appetite Regulatory Peptides in the Hypothalamus of Salmonella-Challenged Broiler Chickens

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Abstract

The effects of dietary Zinc (Zn) supplementation on the gene expression of appetite regulatory peptides were investigated in Salmonella-infected broiler chickens. Broiler chickens (Arbor Acres, 1 day old) were allocated randomly into 24 pens of 10 birds. The chickens from 12 pens were fed with basal diet and the other with basal diet supplemented with Zn (ZnSO4·H2O, 120 mg/kg). At 5 days of age, the chickens were divided into 4 treatments with 6 pens: basal diet; basal diet and Salmonella challenge; Zn-supplemented diet; Zn-supplemented diet and Salmonella challenge. At 42 days of age, the hypothalamus from 6 chickens per treatment (1 chicken per pen) was individually collected for gene expression determination. Results showed that dietary supplementation of Zn reduced the gene expression of hypothalamic ghrelin and tumor necrosis factor alpha (TNF-α) (P < 0.05). Salmonella infection upregulated the messenger RNA (mRNA) levels of hypothalamic neuropeptide Y (NPY) and TNF-α. Zn supplementation and Salmonella inoculation were significantly correlated with the mRNA levels of toll-like receptor 2-1 (P < 0.05). However, neither dietary Zn supplementation nor Salmonella inoculation had significant effect on hypothalamic agouti-related protein, cocaine- and amphetamine-regulated transcript, and pro-opiomelanocortin. This study shows that dietary Zn supplementation promoted orexigenic appetite regulatory peptides and reduced the expression of the inflammatory cytokine TNF-α in the hypothalamus of Salmonella-challenged broilers.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (31172226, 31472115) and the Shandong Modern Agricultural Technology and Industry System (SDAIT-13-011-08). A. Sheikhahmadi wishes to thank the World Academy of Science for their young scientist fellowship grant.

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    Authors and Affiliations

    1. Department of Animal Science, Shandong Agricultural University, Taian, Shandong, 271018, China

      Xiyi Hu, Xianlei Li, Hongchao Jiao, Hai Lin & Zhigang Song

    2. Department of Animal Science, Faculty of Agriculture, University of Kurdistan, Sanandaj, 66177-15175, Iran

      Ardashir Sheikhahmadi

    3. Division Livestock-Nutrition-Quality, Department of Biosystems, KU Leuven, Kasteelpark Arenberg 30, 3001, Leuven, Belgium

      Yufeng Wang

    4. College of Animal Science, China Agricultural University, Beijing, 100094, China

      Bingkun Zhang

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    1. Xiyi Hu
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    Correspondence to Zhigang Song.

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    Xiyi Hu and Ardashir Sheikhahmadi contributed equally to this work.

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    Hu, X., Sheikhahmadi, A., Li, X. et al. Effect of Zinc on Appetite Regulatory Peptides in the Hypothalamus of Salmonella-Challenged Broiler Chickens. Biol Trace Elem Res 172, 228–233 (2016). https://doi.org/10.1007/s12011-015-0582-2

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