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Effects of Dietary Selenium Against Lead Toxicity on mRNA Levels of 25 Selenoprotein Genes in the Cartilage Tissue of Broiler Chicken

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Abstract

The interactions between the essential element selenium (Se) and the toxic element lead (Pb) have been reported extensively; however, little is known about the effect of Se on Pb toxicity and the expression pattern of selenoproteins in the cartilage of chicken. To investigate the effects of Se on Pb toxicity and the messenger RNA (mRNA) expressions of selenoproteins in cartilage tissue, an in vitro study was performed on 1-day-old broiler chickens (randomly allocated into four groups) with diet of different concentration of Se and Pb. After 90 days, the meniscus cartilage and sword cartilage tissue were examined for the mRNA levels of 25 selenoprotein genes. The results showed that Se and Pb influenced the expression of selenoprotein genes in the chicken cartilage tissue. In detail, Se could alleviate the downtrend of the expression of Gpx1, Gpx2, Gpx4, Txnrd2, Txnrd3, Dio1, Dio2, Seli, Selu, Sepx1, Selk, Selw, Selo, Selm, Sep15, Sepnn1, Sels, and Selt induced by Pb exposure in the meniscus cartilage. In the sword cartilage, Se alleviated the downtrend of the expression of Gpx2, Gpx3, Gpx4, Txnrd1, Txnrd2, Dio2, Dio3, Seli, Selh, SPS2, Sepx1, Selk, Selw, Selo, Selm, Sep15, Selpb, Sepn1, and Selt induced by Pb exposure. The present study provided some compensated data about the roles of Se against Pb toxicity in the regulation of selenoprotein expression.

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Acknowledgments

This work was supported by the China Postdoctoral Science Foundation (No. 2012M520702), Startup Foundation for Doctors of Northeast Agricultural University, China (No. 2012RCB92), the Fundamental Research Funds for the Central Universities (Y0201500195), Heilongjiang Provincial Department of Education Science and Technology research project (No. 12541024), Young Talents Project of Northeast Agricultural University (No. 14QC18), and The International Postdoctoral Exchange Fellowship Program (No. 20130006).

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Authors and Affiliations

  1. College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, People’s Republic of China

    H Gao & S. Q. Song

  2. Department of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People’s Republic of China

    C. P. Liu

  3. College of Animal Science and Technology, Northeast Agricultural University, Harbin, 150030, People’s Republic of China

    J. Fu

  4. Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY, USA

    J. Fu

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  1. H Gao
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  2. C. P. Liu
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  4. J. Fu
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Correspondence to S. Q. Song or J. Fu.

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Gao, H., Liu, C.P., Song, S.Q. et al. Effects of Dietary Selenium Against Lead Toxicity on mRNA Levels of 25 Selenoprotein Genes in the Cartilage Tissue of Broiler Chicken. Biol Trace Elem Res 172, 234–241 (2016). https://doi.org/10.1007/s12011-015-0579-x

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  • DOI: https://doi.org/10.1007/s12011-015-0579-x

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