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Arsenic Trioxide Exposure Induces Heat Shock Protein Responses in Cock Livers

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Abstract

Arsenic is a trace element widely found in nature, and there are several forms of arsenic, including the most toxic form of trivalent arsenic. Arsenic trioxide (As2O3) is widespread in nature and tends to accumulate in animals and humans, thus causing great harm. Although the important role of heat shock proteins (HSPs) has been demonstrated in many types of mammals exposed to As2O3, the function of these proteins in poultry, especially in cocks, remains unclear. In this study, we used experimental animals (male chickens), which were fed a diet including 0, 7.5, 15, and 30 mg kg−1 As2O3, respectively, in the control, low, middle, and high groups. The livers were collected after the cocks were treated with arsenic for 30, 60, and 90 days. We detected HSP27, HSP60, HSP70, and HSP90 levels in the livers of the cocks by real-time PCR and HSP60 and HSP70 levels by Western blot. The results showed that the messenger RNA and protein expression of HSPs exposed to As2O3 had obviously increased. These results demonstrated that arsenic toxicity affected the expression of HSP levels in cock livers.

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Acknowledgments

This study was supported by the Heilongjiang Province Natural Science Foundation (Grant No. C2015061).

Conflicts of Interest

The authors declare that they have no competing interests.

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Authors and Affiliations

  1. College of Wildlife Resources, Northeast Forestry University, 26 Hexing Road, Harbin, Heilongjiang Province, 150040, China

    Kexin Zhang, Panpan Zhao, Guangyang Guo, Ying Guo, Siwen Li, Ying He, Xiao Sun, Hongliang Chai, Wen Zhang & Mingwei Xing

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  1. Kexin Zhang
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  2. Panpan Zhao
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  3. Guangyang Guo
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Correspondence to Hongliang Chai, Wen Zhang or Mingwei Xing.

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Kexin Zhang and Panpan Zhao contributed equally to this work.

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Zhang, K., Zhao, P., Guo, G. et al. Arsenic Trioxide Exposure Induces Heat Shock Protein Responses in Cock Livers. Biol Trace Elem Res 170, 459–465 (2016). https://doi.org/10.1007/s12011-015-0487-0

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