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Multispectroscopic Investigation of the Interaction Between two Ruthenium(II) Arene Complexes of Curcumin Analogs and Human Serum Albumin

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Abstract

The interaction between two ruthenium(II) arene complexes of curcumin analogs and human serum albumin (HSA) was systematically investigated by multispectroscopic techniques. The fluorescence spectral results indicated that two complexes quenched the intrinsic fluorescence of HSA through static quenching mode. The quenching constants and the corresponding thermodynamic parameters at different temperatures were calculated. The binding interactions of two complexes with HSA resulted in the complex formation of complex–HSA, and the van der Waals interactions and hydrogen bond interactions played major roles in the complex stabilization. The distances between HSA and two complexes were obtained according to fluorescence resonance energy transfer theory. The site competitive replacement experiments illustrated that two complexes mainly bounded with HSA on site I. The results of synchronous fluorescence spectra, three-dimensional fluorescence spectra, FT–IR spectra, and circular dichroism spectra indicated that the secondary structure of HSA was changed at the present of two complexes. The results of mass spectrometry further validated the binding interaction and the binding number between two complexes and HSA.

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Acknowledgments

This work was financially supported by the National Natural Science Foundation of China (21403039, 21203035, 21261005, 21225313), the Guangxi Natural Science Foundation (2013GXNSFCA019005), the Scientific Research Foundation of Guangxi Provincial Education Department (ZD2014081), the Innovation Project of Guangxi Graduate Education (YCSZ2014186), Open Research Fund Program of the State Key Laboratory of Virology of China (2014KF006), and Collaborative Innovation Center of Southwest Ethnic Medicine (Guangxi Normal University, CICSEM 2013-B6).

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Authors and Affiliations

  1. College of Chemistry and Material Science, Guangxi Teachers Education University, Nanning, 530001, People’s Republic of China

    Shan Huang, Shushu Peng, Fawei Zhu, Xiaolin Lei, Qi Xiao, Wei Su, Chusheng Huang & Lixia Zhang

  2. Collaborative Innovation Center of Southwest Ethnic Medicine, Guangxi Normal University, Guilin, 541004, People’s Republic of China

    Shan Huang, Qi Xiao & Chusheng Huang

  3. State Key Laboratory of Virology, Wuhan University, Wuhan, 430072, People’s Republic of China

    Qi Xiao & Yi Liu

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  1. Shan Huang
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  2. Shushu Peng
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Correspondence to Qi Xiao.

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Huang, S., Peng, S., Zhu, F. et al. Multispectroscopic Investigation of the Interaction Between two Ruthenium(II) Arene Complexes of Curcumin Analogs and Human Serum Albumin. Biol Trace Elem Res 169, 189–203 (2016). https://doi.org/10.1007/s12011-015-0416-2

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