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Organic Selenium Alleviated the Formation of Ethylene Glycol-Induced Calcium Oxalate Renal Calculi by Improving Osteopontin Expression and Antioxidant Capability in Dogs

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Abstract

Twenty one-year-old local male dogs were randomly assigned into four groups (five dogs per group). The control and the ethylene glycol (EG) groups were fed basal diets without and with EG, and the EG + sodium selenite (EG + SS) and EG + selenium yeast (EG + SY) groups were fed basal diets with EG containing SS and SY, respectively. Blood, urine, and renal samples were taken after 18 weeks of feeding. The results showed that compared with the control group, the serum calcium levels and antioxidase activities significantly decreased in the EG group. Serum creatinine, urea nitrogen, and malondialdehyde (MDA) levels and urine calcium and oxalate levels significantly increased. Calcium oxalate crystal deposition and osteopontin (OPN) messenger RNA and protein expression in the renal tissues significantly increased. These changes above in the EG group were reversed within limits by adding selenium in the diets (both EG + SS and EG + SY groups). Further, compared with the EG + SS group, the EG + SY group showed better effects in decreasing the formation of EG-induced calcium oxalate renal calculi and OPN expression and improving antioxidant capability in dogs. It indicates that organic selenium has the potential value to alleviate the formation of EG-induced calcium oxalate renal calculi.

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Acknowledgments

This work was funded by the National Natural Science Foundation of China (NSFC) (Grant numbers 31272627, 31472253) and the Priority Academic Program Development of Jiangsu Higher Education Institutions.

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The authors declare that they have no competing interests.

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    Authors and Affiliations

    1. Institute of Nutritional and Metabolic Disorders in Domestic Animals and Fowls, Nanjing Agricultural University, Nanjing, 210095, China

      Yongwang Liu, Haibin Xu, Wenting Zhong, Qingpeng Shen, Tenghan Zhuang & Kehe Huang

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    1. Yongwang Liu
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    2. Haibin Xu
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    3. Wenting Zhong
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    4. Qingpeng Shen
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    Correspondence to Kehe Huang.

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    Yongwang Liu and Haibin Xu contributed equally to this work.

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    Liu, Y., Xu, H., Zhong, W. et al. Organic Selenium Alleviated the Formation of Ethylene Glycol-Induced Calcium Oxalate Renal Calculi by Improving Osteopontin Expression and Antioxidant Capability in Dogs. Biol Trace Elem Res 168, 392–400 (2015). https://doi.org/10.1007/s12011-015-0373-9

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    • DOI: https://doi.org/10.1007/s12011-015-0373-9

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