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Effects of Sodium Selenite on Aflatoxin B1-Induced Decrease of Ileac T cell and the mRNA Contents of IL-2, IL-6, and TNF-α in Broilers

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Abstract

The aim of this work was to assess the protective effect of sodium selenite on the ileum mucosal immunologic toxicity induced by aflatoxin B1 (AFB1). One hundred and eighty one-day-old healthy male avian broilers were divided into four groups of three replicates and 15 birds per replicate and fed with basal diet (control group), 0.3 mg/kg AFB1 (AFB1 group), 0.4 mg/kg Se (+Se group), and 0.3 mg/kg AFB1 + 0.4 mg/kg Se (AFB1+Se group), respectively. The ileac T-cell subsets were determined by the methods of flow cytometry (FCM), and the mRNA contents of interleukin-2 (IL-2), interleukin-6(IL-6), and tumor necrosis factor-alpha (TNF-α) by quantitative real-time PCR. Compared with those in control group, the percentages of CD3 +, CD3 +CD4 +, CD3 +CD8 + intraepithelial lymphocytes (IELs) and LPLs, the CD4+/CD8+ ratio of IELs, and the mRNA contents of IL-2, IL-6, and TNF-α were decreased in AFB1 group. However, compared with those in AFB1 group, these parameters of AFB1+Se group were increased to be close to those in control group. It was concluded that 0.3 mg/kg AFB1 could reduce the cellular immune function of the ileum mucosa, but 0.4 mg/kg supplemented dietary selenium showed protective effects on AFB1-induced immunologic injury.

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Acknowledgments

This work was supported by the program for Changjiang scholars and university innovative research team (IRT 0848).

Conflict of Interest

The authors have no financial and nonfinancial conflict of interest.

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Authors and Affiliations

  1. Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya’an, Sichuan, 625014, China

    Yang He, Jing Fang, Xi Peng, Hengmin Cui, Zhicai Zuo, Junliang Deng, Zhengli Chen, Weimin Lai, Gang Shu & Li Tang

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  1. Yang He
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  2. Jing Fang
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  3. Xi Peng
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  4. Hengmin Cui
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  7. Zhengli Chen
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  10. Li Tang
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Correspondence to Xi Peng.

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Yang He and Jing Fang contributed equally in this study.

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He, Y., Fang, J., Peng, X. et al. Effects of Sodium Selenite on Aflatoxin B1-Induced Decrease of Ileac T cell and the mRNA Contents of IL-2, IL-6, and TNF-α in Broilers. Biol Trace Elem Res 159, 167–173 (2014). https://doi.org/10.1007/s12011-014-9999-2

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  • DOI: https://doi.org/10.1007/s12011-014-9999-2

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