Abstract
The use of natural remedies and pharmacological mineral supplements for liver disease treatment has a long history. In present study, the levels of selenium (Se) and zinc (Zn) were determined in biological samples (serum and whole blood) of female hepatitis C patients (n = 132), age ranged 30–45 years, before and after 30 days treatment with herbal/pharmaceutical supplements. For comparative study, 128 age-matched female subjects, residing in the same residential areas and have socioeconomic status, were selected as referents. The Se and Zn in supplements, blood, and sera were determined by atomic absorption spectrometry. It was observed that Zn and Se in blood and serum samples of viral hepatitis C (HCV) patients were reduced in the range of 28.6–39 % and 24–36 %, respectively, as compared to those of referents. After herbal/pharmaceutical supplementations, 20.6–25.0 and 9.15–13.2 % of Zn and 10.6–12.1 and 19.6–21.4 % of Se were enhanced in sera and blood samples of HCV patients, respectively. The resulted data indicated that the levels of Se and Zn in addition to some biochemical parameters were improved in HCV patients after herbal/pharmaceutical supplementation. The effects of both supplements were not significantly different (p > 0.05).
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Davis GL, Albright JE, Cook SF, Rosenberg DM (2003) Projecting future complications of chronic hepatitis C in the United States. Liver Transpl 9:331–338
Alter MJ, Margolis HS, Krawczynski K, Judson FN, Mares A, Alexander WJ, Sampliner RE, Meeks EL, Beach MJ (1992) The natural history of community acquired hepatitis C in the United States. N Engl J Med 327:1899–1905
Dienstag JL, McHutchison JG (2006) American gastroenterological association technical review on the management of hepatitis C. Gastroenterology 130:231–264
Machida K, Cheng KT, Lai CK, Jeng KS, Sung VM, Lai MM (2006) Hepatitis C virus triggers mitochondrial permeability transition with production of reactive oxygen species, leading to DNA damage and STAT3 activation. J Virol 80:7199–207
Pal S, Polyak SJ, Bano N, Qiu WC, Carithers RL, Shuhart M, Gretch DR, Das A (2010) Hepatitis C virus induces oxidative stress, DNA damage and modulates the DNA repair enzyme NEIL1. J Gastroenterol Hepatol 25:627–634
Goldhaber SB (2003) Trace elements risk assessment: essentiality vs toxicity. Regul Toxicol Pharmacol 38:232–42
Bhaskaram P (2002) Micronutrient malnutrition, infection, and immunity: an overview. Nutr Rev 60:40–45
Prasad AS, Bao B, Beck FW, Kucuk O, Sarkar FH (2004) Antioxidant effect of zinc in humans. Free Radic Biol Med 37:1182–1190
Powell SR (2000) The antioxidant properties of zinc. J Nutr 130:1447–1454
Krebs NE, Hambidge KM (2001) Zinc metabolism and homeostasis: the application of tracer techniques to human zinc physiology. Biometals 14:397–412
Kalkan A, Bulut V, Avci S, Celik I, Bingol NK (2002) Trace elements in viral hepatitis. J Trace Elem Med Biol 16:227–230
Gur G, Bayraktar Y, Ozer D, Ozdogan M, Kayhan B (1998) Determination of hepatic zinc content in chronic liver disease due to hepatitis B virus. Hepato-Gastroenterol 45:472–476
Stamoulis I, Kouraklis G, Theocharis S (2007) Zinc and the liver: an active interaction, review article. Dig Dis Sci 52:1595–1612
Maret W (2003) Cellular zinc and redox states converge in the metallothionein/thionein pair. J Nutr 133:1460–1462
Demirci S, Kutluhan S, Naziroglu M, Uguz AC, Yurekli VA, Demirci K (2013) Effects of selenium and topiramate on cytosolic Ca(2+) influx and oxidative stress in neuronal PC12 cells. Neurochem Res 38:90–7
Naziroglu M (2009) Role of selenium on calcium signaling and oxidative stress-induced molecular pathways in epilepsy. Neurochem Res 34:2181–91
Thomson CD (2004) Assessment of requirements for selenium and adequacy of selenium status: a review. Eur J Clin Nutr 58:391–402
Alaejos MS, Rornero CD (1995) Analysis of selenium in body fluids: a review. Chem Rev 95:227–257
Kaufman PB, Csake LJ, Warber S, Duke JA, Brielmann HL (eds) (1999) Natural products from plants. CRC, Boca Raton
Singh V, Garg AN (1997) Availability of essential trace elements in Ayurvedic Indian medicinal herbs using instrumental neutron activation analysis. Appl Radiat Isot 48:97–101
WHO (1998) Regulatory situation of herbal medicines. A worldwide review. Who, Geneva, pp 1–5
Basgel S, Erdemoglu SB (2006) Determination of mineral and trace elements in some medicinal herbs and their infusions consumed in Turkey. Sci Total Environ 359:82–89
Huie CW (2002) A review of modern sample-preparation techniques for the extraction and analysis of medicinal plants. Anal Bioanal Chem 373:23–30
Choudhary F, Iqbal Z, Khan T, Ashraf M (2005) Trace metals analysis in selected pharmaceutical multimineral formulations. Pak J Pharm Sci 18:40–43
Food and Nutrition Board (2000) Institute of Medicine Dietary reference intakes for vitamin C, vitamin E, selenium, and carotenoids. The National Academies Press, Washington
Roy A, Evers SE, Campbell MK (2012) Dietary supplement use and iron, zinc and folate intake in pregnant women in London, Ontario. Chron Dis Inj Can 32:76–83
Bahadur A, Chaudhry Z, Jan G, Danish M, Rehman A, Ahmad R et al (2011) Nutritional and elemental analyses of some selected fodder species used in traditional medicine. Afr J Pharm Pharmacol 5(8):1157–1161
Bukhsh E, Malik SA, Ahmad SS (2007) Estimation of nutritional value and trace elements content of Carthamus oxyacantha, Eruca sativa and Plantago ovata. Pak J Bot 39(4):1181–1187
Nakayama A, Fukuda H, Ebara M, Hamasaki H, Nakajima K, Sakurai H (2002) A new diagnostic method for chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma based on serum metallothionein, copper, and zinc levels. Biol Pharm Bull 25:426–431
Spallholz JE, Boylan LM, Larsen HS (1990) Advances in understanding selenium’s role in the immune system. Ann NY Acad Sci 587:123–139
Taylor EW, Nadimpalli RG, Ramanathan CS (1997) Genomic structures of viral agents in relation to the biosynthesis of selenoproteins. Biol Trace Elem Res 56:63–91
Al-Bader A, Abul H, Hussain T, Al-Moosawi M, Mathew TC, Dashti H (1998) Selenium and liver cirrhosis. Mol Cell Biochem 185:1–6
Ding M, Potter JJ, Liu X, Torbenson MS, Mezey E (2010) Selenium supplementation decreases hepatic fibrosis in mice after chronic carbon tetrachloride administration. Biol Trace Elem Res 133:83–97
Fota-Markowska H, Przybyla A, Borowicz I, Modrzewska R (2002) Serum zinc (Zn) level dynamics in blood serum of patients with acute viral hepatitis B and early recovery period. Ann Univ Mariae Curie Sklodowska 57:201–209
Grungreiff K (2002) Zinc in liver disease. J Trace Elem Exp Med 15:67–78
Shi JY, Chen YX, Huang YY, He W (2004) SRXRF microprobe as a technique for studying elements distribution in Elsholtzia splendens. Micron 35:557–564
Alberino F, Gatta A, Amodio P, Merkel C, Pascoli LD, Boffo G et al (2001) Nutrition and survival in patients with liver cirrhosis. Nutrition 17:445–450
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The authors thank the Higher Education Commission, Pakistan, for providing financial support to the National Center of Excellence in Analytical Chemistry, Sindh University Jamshoro, Pakistan, which is sponsoring this project.
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Kolachi, N.F., Kazi, T.G., Afridi, H.I. et al. Effects of Selenium and Zinc Status in Biological Samples of Hepatitis C Patient After Herbal and Pharmaceutical Supplements. Biol Trace Elem Res 152, 187–194 (2013). https://doi.org/10.1007/s12011-013-9617-8
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DOI: https://doi.org/10.1007/s12011-013-9617-8