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Effects of Different Calcium, Magnesium, and Zinc Concentrations Supplemented on Hepatopancreatic Cell Proliferation of Kuruma Prawn, Penaeus japonicus

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Abstract

The effects of different calcium (Ca2+), magnesium (Mg2+), and zinc (Zn2+) concentrations supplemented on hepatopancreatic cell proliferation of kuruma prawn, Penaeus japonicus was studied. The culture system consists of medium 199 (M 199) supplemented with 0.060 mol/L NaCl, 1.011 g/L glucose, 1,000 UI/ml penicillin, 1,000 μg/ml treptomycin, heat inactivated fetal calf serum (FCS) 20% for primary cell culture and 10% for subculture. The RNA/DNA ratio of the cell cultures was measured. The results show that the cell division of hepatopancreatic cells of P. japonicus was enhanced by the optimal concentration of inorganic salt (Ca2+, 1.0 g/L; Mg2+, 5.0 g/L; Zn2+, 80 μg/L). The hepatopancreatic cell culture system and improved culture conditions described here will be very useful for in vitro experiments to study viruses responsible for infections in shrimp leading to tremendous economic losses.

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Acknowledgments

We wish to thank Dr. Jomo MacDermott, Prof. Xin-ping Chang, and Mr. Chris Albright for editing this manuscript. We are grateful for the constructive comments of the reviewers. This research was supported by National Major Project on Water Pollution Control and Management Technology (2009ZX07528-001) of P.R. China; Projects of Hebei Province Fishery Institute (Ji Yu Ke: 20110403); Doctoral fund of Hebei University(2011). This work funded by China Scholarship Council.

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Authors and Affiliations

  1. South China Institute of Environmental Sciences, Ministry of Environmental Protection, Guangzhou, People’s Republic of China

    Hongwei Wang & Kaiming Li

  2. Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou, People’s Republic of China

    Hongwei Wang

  3. College of Life Science, Hebei University, Baoding, People’s Republic of China

    Hongwei Wang, Likun Yang & Na Zhu

  4. Laboratory of Environmental Toxicology and Aquatic Ecology, Ghent University, J. Plateaustraat 22, B-9000, Ghent, Belgium

    Hongwei Wang

  5. College of Quality and Technical Supervision, Hebei University, Baoding, People’s Republic of China

    Duanbo Cai

  6. Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, People’s Republic of China

    Haiming Xu

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  1. Hongwei Wang
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  2. Duanbo Cai
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  3. Kaiming Li
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  5. Likun Yang
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Correspondence to Kaiming Li.

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Wang, H., Cai, D., Li, K. et al. Effects of Different Calcium, Magnesium, and Zinc Concentrations Supplemented on Hepatopancreatic Cell Proliferation of Kuruma Prawn, Penaeus japonicus . Biol Trace Elem Res 148, 198–202 (2012). https://doi.org/10.1007/s12011-012-9366-0

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  • DOI: https://doi.org/10.1007/s12011-012-9366-0

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