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Excess Dietary Sodium Selenite Alters Apoptotic Population and Oxidative Stress Markers of Spleens in Broilers

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Abstract

Three hundred 1-day-old avian broilers were fed on a basic diet (0.2 mg/kg selenium) or the same diet amended to contain 1, 5, 10, and 15 mg/kg selenium supplied as sodium selenite (n = 60/group). In comparison with those of 0.2 mg/kg selenium group, the percentages of annexin V-positive splenocytes were increased in 5, 10, and 15 mg/kg selenium groups. TUNEL assay revealed that apoptotic cells with brown-stained nuclei distributed within the red pulp and white pulp of the spleens with increased frequency of occurrence in 10 and 15 mg/kg selenium groups in comparison with that of 0.2 mg/kg Se group. Sodium selenite-induced oxidative stress in spleens of chickens was evidenced by decrease in glutathione peroxidase, superoxide dismutase, and catalase activities and increase in malondialdehyde contents. The results indicate that excess dietary selenium in the range of 5–15 mg/kg of feed causes oxidative stress, which may be mainly responsible for the increased apoptosis of splenocytes in chickens.

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Acknowledgment

This study was supported by Program for Changjiang Scholars and Innovative Research Team in University (IRT 0848) and Education Department of Sichuan Province (09ZZ017).

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Authors and Affiliations

  1. College of Veterinary Medicine, Sichuan Agricultural University, Ya’an, Sichuan, 625014, China

    Xi Peng, Hengmin Cui, Yaxin He, Wei Cui, Jing Fang, Zhicai Zuo, Junliang Deng, Kangcheng Pan, Yi Zhou & Weimin Lai

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  1. Xi Peng
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  2. Hengmin Cui
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  3. Yaxin He
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  4. Wei Cui
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  7. Junliang Deng
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  8. Kangcheng Pan
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  9. Yi Zhou
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  10. Weimin Lai
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Correspondence to Hengmin Cui.

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Peng, X., Cui, H., He, Y. et al. Excess Dietary Sodium Selenite Alters Apoptotic Population and Oxidative Stress Markers of Spleens in Broilers. Biol Trace Elem Res 145, 47–51 (2012). https://doi.org/10.1007/s12011-011-9160-4

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  • DOI: https://doi.org/10.1007/s12011-011-9160-4

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