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Manganese Regulates Manganese-Containing Superoxide Dismutase (MnSOD) Expression in the Primary Broiler Myocardial Cells

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Abstract

Previous studies showed that dietary manganese can increase the MnSOD mRNA expression in a dose-dependent manner in the heart of broilers. In order to explore the specific mechanism of the MnSOD expression induced by manganese, a model of MnSOD expression was developed with primary cultured broiler myocardial cells. The objective of the present study was to investigate whether the model was working or not and to determine how manganese affects the expression of the enzyme in broiler myocardial cells in vitro. In experiment 1, various amount of manganese (0, 0.25, 0.5, 1, 2, and 4 mM) were added into the cultures for 24-h incubation to investigate MnSOD expression and for 0-, 6-, 12-, 24-, 36-, and 48-h incubation to measure the cell viability. In experiment 2, the most suitable Mn supplementation based on the results of experiment 1 was added into cultures for 6-, 12-, 24-, and 48-h incubation. The results showed that MnSOD mRNA, MnSOD protein, and MnSOD activity were induced by manganese in dose- and time-dependent manner. Manganese regulates MnSOD expression not only at transcriptional level but also at translational and/or posttranslational levels.

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Abbreviations

MnSOD:

Manganese-containing superoxide dismutase

mETC:

Mitochondrial electron transport chain

ROS:

Reactive oxygen species

TNF-α:

Tumor necrosis factor-α

LPS:

Lipopolysaccharides

PBS:

Phosphate-buffered saline

CCK-8:

Cell counting Kit-8

PCR:

Polymerase chain reaction

MnSODp:

MnSOD protein

BSA:

Bovine serum albumin

SDS:

Sodium dodecyl sulfate

ECL:

Enhanced chemiluminescence

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Acknowledgments

This study was supported by the Key Program of the National Natural Science Foundation of China (Project No. 30530570), China Agriculture Research System (Project No. CARS-42; Beijing, P.R. China), and the National Key Technology Research and Development Program (Project No. 2011BAD26B04; Beijing, P.R. China).

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Authors and Affiliations

  1. Institute of Animal Nutrition, Sichuan Agricultural University, Ya’an, 625014, China

    Tianquan Gao & Keying Zhang

  2. Mineral Nutrition Research Division, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, China

    Sufen Li & Xugang Luo

  3. State Key Laboratory of Animal Nutrition, Beijing, 100193, China

    Sufen Li & Xugang Luo

  4. College of Animal Science and Technology, Northwest A & F University, Yangling, Shaanxi, 712100, China

    Funing Wang

  5. Department of Animal Science, Hebei Normal University of Science and Technology, Qinhuangdao, 066000, China

    Sufen Li

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  1. Tianquan Gao
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  2. Funing Wang
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  3. Sufen Li
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  4. Xugang Luo
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  5. Keying Zhang
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Corresponding authors

Correspondence to Xugang Luo or Keying Zhang.

Additional information

Tianquan Gao and Funing Wang contributed equally to this work.

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Gao, T., Wang, F., Li, S. et al. Manganese Regulates Manganese-Containing Superoxide Dismutase (MnSOD) Expression in the Primary Broiler Myocardial Cells. Biol Trace Elem Res 144, 695–704 (2011). https://doi.org/10.1007/s12011-011-9093-y

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  • DOI: https://doi.org/10.1007/s12011-011-9093-y

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