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Cardiac Effects of Magnesium Sulfate Pretreatment on Acute Dichlorvos-Induced Organophosphate Poisoning: An Experimental Study in Rats

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Abstract

Although atropine and oximes are traditionally used in the management of organophosphate poisoning, investigations have been directed to finding additional therapeutic approaches. Thus, the aim of this study was to evaluate the cardiac effects of magnesium sulfate pretreatment on dichlorvos intoxication in rats. Rats were randomly divided into three groups as control, dichlorvos, and magnesium sulfate groups. After 6 h of dichlorvos or corn oil (as a vehicle) injection, venous blood samples were collected, and cardiac tissue samples were obtained. Biochemical analyses were performed to measure some parameters on serum and cardiac tissue. Immunohistochemical analyses of apoptosis and inducible nitric oxide (NO) synthase showed no change in cardiac tissue. Serum cholinesterase levels were markedly depressed with dichlorvos, and further suppressed markedly with magnesium sulfate pretreatment. Although we have demonstrated that serum NO levels in dichlorvos and magnesium sulfate groups were lower than the control group, cardiac tissue NO levels in magnesium sulfate group were higher than the other two groups. Mortality was not significantly affected with magnesium sulfate pretreatment. Uncertainty still persists on the right strategies for the treatment of organophosphate acute poisoning; however, it was concluded that our results do not suggest that magnesium sulfate therapy is beneficial in the management of acute dichlorvos-induced organophosphate poisoning, and also further studies are required.

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Acknowledgment

This study was supported by a project (SBAG-HD-112, 106 S007) from the Scientific and Technological Research Council of Turkey (TUBITAK).

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Authors and Affiliations

  1. Department of Emergency Medicine, Kayseri Training and Research Hospital, Kayseri, Turkey

    Nurullah Gunay

  2. Department of Emergency Medicine, Faculty of Medicine, Cukurova University, Adana, Turkey

    Zeynep Kekec

  3. Gaziantep School of Health, and Department of Physiology, Faculty of Medicine, Gaziantep, Turkey

    Seniz Demiryurek

  4. Department of Emergency Medicine, Twenty-Five December State Hospital, Gaziantep, Turkey

    Ataman Kose

  5. Department of Biochemistry and Clinical Biochemistry, Faculty of Medicine, University of Gaziantep, Gaziantep, Turkey

    Emine S. Namiduru

  6. Department of Biochemistry and Clinical Biochemistry, Gaziantep Pediatric Hospital, Gaziantep, Turkey

    Nahide E. Gunay

  7. Department of Pathology, Faculty of Medicine, University of Gaziantep, Gaziantep, Turkey

    Ibrahim Sari

  8. Department of Pharmacology, Faculty of Medicine, University of Gaziantep, Gaziantep, Turkey

    Abdullah T. Demiryurek

Authors
  1. Nurullah Gunay
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  2. Zeynep Kekec
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  3. Seniz Demiryurek
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  4. Ataman Kose
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  5. Emine S. Namiduru
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  6. Nahide E. Gunay
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  7. Ibrahim Sari
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  8. Abdullah T. Demiryurek
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Correspondence to Nurullah Gunay.

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Gunay, N., Kekec, Z., Demiryurek, S. et al. Cardiac Effects of Magnesium Sulfate Pretreatment on Acute Dichlorvos-Induced Organophosphate Poisoning: An Experimental Study in Rats. Biol Trace Elem Res 133, 227–235 (2010). https://doi.org/10.1007/s12011-009-8418-6

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  • DOI: https://doi.org/10.1007/s12011-009-8418-6

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