Abstract
Taking into account the importance role of lipid peroxidation and antioxidants in the prevention and incidence of cancer, the present study was carried out to determine oxidative stress, serum total antioxidant (TAS), and vitamin C levels in cancer patients. Malondialdehyde(MDA), total antioxidant status, and vitamin C levels of 57cancer patients aged 19–80 years and 22 healthy subjects (control group) aged 22–76 years were evaluated. Serum concentrations of MDA as thiobarbitaric acid complexes were measured by fluorometry method, the serum TAS by using commercial test kits from Randox Laboratories, and vitamin C by using spectrocolorimetric method. The mean serum MDA concentrations of all cancer groups except lung cancer were significantly higher than control group (P < 0.004). The mean total antioxidant status was insignificantly higher than control group. The mean serum vitamin C level was significantly lower in patients as compared to the healthy subjects (PV < 0.0001). In conclusion, an alteration in the lipid peroxidation with concomitant changes in antioxidant defense system in cancer patients may be due to excessive oxidative stress. Serum low levels of vitamin C in the different type of cancer patients in spite of adequate daily intake may be due to increased utilization to scavenge lipid peroxides as well as their sequestration by tumor cells.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Jemal A, Thomas A, Murray T, Thun M (2002) Cancer statistics, CA. Cancer J Clin 52:23–47
Howe HL, Wingo PA, Thun MJ, Ries LA, Rosenberg HM, Feigal EG (2001) Annual report to the nation on the status of cancer [1973 through 1998], featuring cancers with recent increasing trends. J Natl Cancer Inst 93:824–842
Valko M, Izakovic M, Mazur M, Rhodes CJ, Telser J (2004) Role of oxygen radicals in DNA damage and cancer incidence. Mol Cell Biochem 266:37–56
Yeh CC, Hou MF, Tasi S, Lin SK, Hsiao JK, Huang JC et al (2005) Superoxide anion radical, lipid peroxidation and antioxidant status in the blood of patients with breast cancer. Clin Chim Acta 361:104–111
Pathak SK, Sharma AR, Steward WP, Mellon JK, Griffiths TRL, Gescher AJ (2005) Oxidative stress and cyclooxygenase activity in prostate carcinogenesis: targets for chemopreventive strategies. Eur J Cancer 41:61–70
Casaril M, Corso F, Corrocher R (1991) Free radicals in human pathology. Recenti Prog Med 82:39–44
Aas PA, Otterlei M, Falnes PO, Vågbø CB, Skorpen F, Akbari M et al (2003) Human and bacterial oxidative demethylases repair alkylation damage in both RNA and DNA. Nature 421:859–863
Das UN (2002) A radical approach to cancer. Med Sci Monit 8:79–92
Ames BN (1998) Micronutrients prevent cancer and delay aging. Toxicol Lett 102/103:5–18
World Cancer Research Fund/American Institute for Cancer Research (1997) Food research and the prevention of cancer: a global perspective. American Institute for Cancer Research, Washington, DC
Block G, Dietrich M, Norkus EP, Morrow JD, Hudes M, Caan B (2002) Factors associated with oxidative stress in human populations. Am J Epidemiol 156:274–85
Wasowicz W, Neve J, Peretz A (1993) Optimized steps in fluoromertic determination of thiobarbituric acid-reactive substances in serum importance of extraction PH and influence of sample preservation and storage. Clin Chem 39:2522–2526
Burtis CA, Ashwood ER (1999) Tietz textbook of clinical chemistry. Saunders, Philadelphia
Clancy KJ, Davidson NJ (2000) The biology of breast carcinoma. Cancer 97:825–833
Rushmore TH, Morton MR, Pickett CB (1992) The antioxidant response element. J Biol Chem 66:11632–11639
Senthil K, Aranganathan S, Nalini N (2004) Evidence of oxidative stress in the circulation of ovarian cancer patients. Clin Chim Acta 399:27–32
Manju V, Kalaivaani SJ, Nalini N (2002) Circulation lipid peroxidation and antioxidant status in cervical cancer patients: a case–control study. Clin Biochem 35:621–625
Beevi SS, Rasheed AM, Geetha A (2004) Evaluation of oxidative stress and nitric oxide levels in patients with oral cavity cancer. Jpn J Clin Oncol 34:379–385
Hristozov D, Gadjeva V, Vlykova T, Dimiitrov G (2001) Evaluation of oxidative stress in patients with cancer. Arch Physiol Biochem 109:331–336
Skrzydlewska E, Sullkowski S, Koda M, Zalewski B, Kanczuga-Koda L, Sulkowska M (2005) Lipid peroxidation and antioxidant status in colorectal cancer. World J Gastroenterol 11:403–406
Kumaraguruparan R, Subapriya R, Kabalimoorthy J, Nagini S (2002) Antioxidant profile in the circulation of patients with fibro adenoma and adenocarcinoma of the breast. Clin Biochem 35:275–279
Prior RL, Cao G (1999) In vivo antioxidant capacity: comparison of different analytical methods. Free Rad Med 27:1173–1181
Khanzode SS, Khanzode SD, Dakhle GN (2003) Serum and plasma concentration of oxidant and antioxidants in patients of Helicobacter pylori gastritis and its correlation with gastric cancer. Cancer Lett 195:27–31
Levine M, Katz A, Padayatty S (1999) Vitamin C. In: Shils ME, Olson JA, Shike M, Ross AC (eds) Modern nutrition in health and disease. Williams & Wilkins, Baltimore, pp 507–524
Lee WK, Lee HJ, Surh YJ, Lee CY (2003) Vitamin C and cancer chemoprevention. Am J Clin Nutr 78:1074–1078
Acknowledgments
Financial support of Tabriz University of Medical Sciences is gratefully acknowledged.
Author information
Authors and Affiliations
Corresponding authors
Rights and permissions
About this article
Cite this article
Mahdavi, R., Faramarzi, E., Seyedrezazadeh, E. et al. Evaluation of Oxidative Stress, Antioxidant Status and Serum Vitamin C Levels in Cancer Patients. Biol Trace Elem Res 130, 1–6 (2009). https://doi.org/10.1007/s12011-008-8309-2
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12011-008-8309-2