Abstract
The effect of age and gender on major, minor, and trace element contents in the intact rib bone of 80 relatively healthy 15–55-year-old women and men was investigated. Contents or upper limit of contents of 16 chemical elements in the rib bone were determined by inductively coupled plasma atomic emission spectrometry (ICP-AES). Mean values (M ± SΕΜ) for the mass fraction of Ba, Ca, Cu, Fe, K, Li, Mg, Na, P, S, Sr, and Zn (milligram per kilogram of dry bone) were as follows: 2.54 ± 0.16, 171,400 ± 4,050, 1.35 ± 0.22, 140 ± 11, 1,874 ± 71, 0.049 ± 0.011, 2,139 ± 38, 5,378 ± 88, 75,140 ± 1,660, 1,881 ± 51, 291 ± 20, and 92.8 ± 1.5, respectively. The upper limits of contents of Al, B, Mn, and V were <7.20, <0.65, <0.36, and <0.03, respectively. Statistically significant tendency for the Ca, Mg, and P content to decrease with age was found in the human rib bone, regardless of gender. The mass fraction of Fe in the male rib bone increases with age. It was shown that higher Ca, Mg, Na, P, and Sr mass fractions as well as lower Fe content were typical of female ribs as compared to those in male ribs.
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Beattie JH, Avenell A (1992) Trace element nutrition and bone metabolism. Nutr Res Rev 5:167–188
Lewinnek GE, Kelsey J, White AA et al (1980) The significance and comparative analysis of the epidemiology of hip fractures. Clin Orthop 152:35–43
Saltman P, Strause L (1993) The role of trace elements in osteoporosis. J Am Coll Nutr 12:384–389
Bowen HJM, Gibbons D (1963) Radioactivation analysis. The Clarendon, Oxford
Bowen HJM (1979) Environmental chemistry of the elements. Academic, London
Zwanziger H (1989) The multielemental analysis of bone: a review. Biol Trace Elem Res 19:195–232
Iyengar GV, Kollmer WE, Bowen HGM (1978) The elemental composition of human tissues and body fluids. A compilation of values for adults. Verlag Chemie, Weinheim
Iyengar GV, Tandon L (1999) Minor and trace elements in human bones and teeth. IAEA (NAHRES-39), Vienna
Grynpas MD, Pritzker KPH, Hancock RGV (1987) Neutron activation analysis of bulk and selected trace elements in bone using low flux SLOWPOKE reactor. Biol Trace Elem Res 13:333–344
Zaichick V (1997) Sampling, sample storage and preparation of biomaterials for INAA in clinical medicine, occupational and environmental health, in Harmonization of Health-Related Environmental Measurements Using Nuclear and Isotopic Techniques, IAEA, pp 123–133
Zaichick V, Zaichick S (1998) INAA application for the assessment of chemical element losses under dry ashing of biological materials, in International Conference on Nuclear Analytical Methods in the Life Sciences pp 95–97
Zaichick V (2004) Losses of chemical elements in biological samples under the dry aching process. Trace Elem Med 5(3):17–22
Kehoe RA, Cholak J, Story RV (1940) A spectrochemical study of the normal ranges of concentrations of certain trace metals in biological materials. J Nutr 19:579–588
Yoshinaga J, Suzuki T, Morita M (1989) Sex- and age-related variation in elemental concentrations of contemporary Japanese ribs. Sci Total Environ 79:209–221
Hamilton EI (1979) The chemical elements and man. Charles C Thomas, Springfield
Schroeder HA, Tipton IH, Nason AP (1972) Trace metals in man: strontium and barium. J Chron Dis 25:491–517
Samudralwar DL, Robertson JD (1993) Determination of major and trace elements in bones by simultaneous PIXE/PIGE analysis. J Radioanal Nucl Chem 169:259–267
Suzuki Y (1979) The normal levels of fluorine in the bone tissue of Japanese subjects. Tohoku J Exp Med 1291:327–336
Yoshinaga J, Suzuki T, Morita M et al (1995) Trace elements in ribs of elderly people and elemental variation in the presence of chronic diseases. Sci Total Environ 162:239–252
Nusbaum RE, Butt EM, Gilmour TC et al (1965) Relation of air pollution to trace metals in bone. Arch Environ Health 10:227–232
Takata MK, Saiki M, Sumita NM et al (2005) Trace element determinations in human cortical and trabecular bones. J Radioanal Nucl Chem 264:5–8
Panday VK (1981) Certain trace elements in normal human bone. Bull Radiat Prot 4:11–15
Crawford MD, Crawford T (1969) Lead content of bones in a soft and hard water area. Lancet 1(No. 7597):699–701
Anke M, Schneider H-J, Grun M et al (1978) Die Diagnose des Mangan-, Zink- und Kupfermangels und der Kadmiumbelastung. Zbl Pharm 117:688–705
Woodard HG, White DR (1982) Bone models for use in radiotherapy dosimetry. Brit J Radiol 55:277–282
Schneider H-J, Anke M (1971) Die Abhangigkeiten des Kalzium-, Phosphor- und Mangangehaltes verschiedener Organe des Menschen. Arch Exper Vet Med 25:787–792
Hamilton EI, Minski MJ (1972/1973) Abundance of the chemical elements in man’s diet and possible relations with environmental factors. Sci Total Environ 1:375–394
Brätter P, Gawlik D, Lausch J et al (1977) On the distribution of the trace elements in human skeletons. J Radioanal Chem 37:393–403
Byrne AR, Kosta L (1978) Vanadium in foods and in human body fluids and tissues. Sci Total Environ 10:17–30
Sumino K, Hayakawa K, Shibata T et al (1975) Heavy metals in normal Japanese tissues. Arch Environ Health 30:487–494
Koch HJ, Smith ER, McNeely J (1957) Analysis of trace elements in human tissue. II. The lymphomatous disease. Cancer 10:151–160
Zaichick V, Dyatlov A, Zaihick S (2000) INAA application in the age dynamics assessment of major, minor, and trace elements in the human rib. J Radioanal Nucl Chem 244:189–193
Tzaphlidou M, Zaichick V (2003) Calcium, phosphorus, calcium–phosphorus ratio in rib bone of healthy humans. J Biol Trace Elem Res 93:63–74
Zaichick V (2008) Neutron activation analysis of trace element contents in the rib bone of healthy men, in 9th International Conference on Nuclear Analytical Methods in the Life Sciences—NAMLS-9, Lisbon, Portugal, p A033
Tipton IH, Johns JC, Boyd M (1968) The variation with age of elemental concentrations in human tissue, in Proceedings First International Congress of Radiation Protection, Pergamon, Elmsford, NY, p 759
Anke M, Latunde-Dada O, Arnhold W et al (1999) The influence of age, sex and cadmium exposure on the ash, calcium, phosphorus, trace element and ultra trace element content in skeleton, kidneys and liver of humans, in Advances in the prevention of environmental cadmium pollution and countermeasures, Eiko Laboratory, Kanazawa Germany.
Anke M, Glei M, Mulleret R et al (2001) Macro, trace and ultratrace element intake depending on the geological origin of the habitat, time, sex and form of diet, in Biochemistry and Geochemical Ecology, Moscow, pp. 235–263
Patti F, Garcet M, Jeanmaire L (1984) Concentration of stable zinc in human bones. Determination by X-ray fluorescence spectrography. Sci Total Environ 39:71–79
Schuhmacher M, Domingo JL, Llobet JM et al (1992) Levels of same trace elements in autopsy tissues from subjects living in Tarragona province, Spain. In: Anastassopoulou J, et al (eds) John Libbey Eurotext, Paris, pp 430–431
Sowden EM, Stitch SR (1957) Trace elements in human tissue. 2. Estimation of the concentrations of stable strontium and barium in human bone. Biochem J 67:104–109
Tanaka G, Kawamura H, Nomura E (1981) Distribution of strontium in the skeleton and in the mass of mineralized bone. Health Phys 40:601–614
Vuorinen HS, Pihlman S, Mussalo-Rauhamaa H et al (1996) Trace and heavy metal analyses of a skeletal population representing the town people in Turku (ABO), Finland in the 16th–17th centuries: with special reference to gender, age and social background. Sci Total Environ 177:145–160
Acknowledgments
We are grateful to Dr. Sergey Moiseev, Hospital Department of Forensic Medicine, Obninsk for supplying rib samples and Ms. Irina Moskvina, Institute of Microelectronics Technology and High Purity Materials, Chernogolovka for the assistance in the ICP-AES measurements.
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Zaichick, V., Zaichick, S., Karandashev, V. et al. The Effect of Age and Gender on Al, B, Ba, Ca, Cu, Fe, K, Li, Mg, Mn, Na, P, S, Sr, V, and Zn Contents in Rib Bone of Healthy Humans. Biol Trace Elem Res 129, 107–115 (2009). https://doi.org/10.1007/s12011-008-8302-9
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DOI: https://doi.org/10.1007/s12011-008-8302-9