Green Synthesis and Characterisation of Silver Nanoparticles Using Cassia tora Seed Extract and Investigation of Antibacterial Potential

Abstract

Nanoparticle research is fascinating and getting hold of consequences due to the wide variety of applications in the biomedical field. Green synthesis of nanoparticles is a cost-effective and eco-friendly approach. It can be synthesised using fungi, algae, plant, yeast, bacteria, microbial enzymes etc. Our current research study focuses on the green synthesis of silver nanoparticles using seed extract of Cassia tora. The colour change from yellow to red colour confirms the formation of silver nanoparticles. The synthesised silver nanoparticles were characterised by Ultraviolet–Visible spectroscopy, Fourier-transform infrared (FTIR), X-ray diffraction analysis (XRD), Scanning Electron Microscopy (SEM) and antibacterial efficacy against three different strains were analysed. The surface plasmon resonance of synthesised AgNPs using Cassia tora seed extract shows maximum absorption peak at 423 nm in UV–visible spectroscopy. X-ray diffraction displays the crystalline nature of synthesised AgNPs and they exhibited four distinct peaks at 36.69°, 42.92°, 63.27° and 76.46°. The particle size of synthesised AgNPs observed through SEM was found to be 55.80 nm, 58.97 nm, 61.06 nm, 63.26 nm and 64.80 nm. S.aureus exhibited maximum zone of inhibition of 12 mm and 13 mm when treated with 25 and 50 μl of the synthesised nanoparticles. Thus, the green synthesised silver nanoparticle using Cassia tora seed extract proved to possess strong anti-bacterial activity.