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Enhanced Tolerance of Spathaspora passalidarum to Sugarcane Bagasse Hydrolysate for Ethanol Production from Xylose

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Abstract

During the pretreatment and hydrolysis of lignocellulosic biomass to obtain a hydrolysate rich in fermentable sugars, furaldehydes (furfural and hydroxymethylfurfural), phenolic compounds, and organic acids are formed and released. These compounds inhibit yeast metabolism, reducing fermentation yields and productivity. This study initially confirmed the ability of Spathaspora passalidarum to ferment xylose and demonstrated its sensibility to the inhibitors present in the hemicellulosic sugarcane bagasse hydrolysate. Then, an adaptive laboratory evolution, with progressive increments of hydrolysate concentration, was employed to select a strain more resistant to hydrolysate inhibitors. Afterward, a central composite design was performed to maximize ethanol production using hydrolysate as substrate. At optimized conditions (initial cell concentration of 30 g/L), S. passalidarum was able to produce 19.4 g/L of ethanol with productivity, yield, and xylose consumption rate of 0.8 g/L.h and 0.4 g/g, respectively, in a sugarcane bagasse hemicellulosic hydrolysate. A kinetic model was developed to describe the inhibition of fermentation by substrate and product. The values obtained for substrate saturation and inhibition constant were Ks = 120.4 g/L and Ki = 1293.4 g/L. Ethanol concentration that stops cell growth was 30.1 g/L. There was an agreement between simulated and experimental results, with a residual standard deviation lower than 6%.

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Data Availability

The authors confirm that the data and materials supporting the findings of this article are available within the article and from the corresponding author (S. B. G.) upon reasonable request.

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Authors and Affiliations

  1. Embrapa Agroenergia, Parque Estação Biológica – PqEB s/no, W3 Norte (final), Brasília, DF, 70770-901, Brazil

    Thályta F. Pacheco, Breno R. C. Machado, João R. M. Almeida & Sílvia B. Gonçalves

  2. CIETI, School of Engineering (ISEP), Polytechnic Institute of Porto (P. Porto), R. Dr. António Bernardino de Almeida, 431, 4249-015, Porto, Portugal

    Wilson G. de Morais Júnior

Authors
  1. Thályta F. Pacheco
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  2. Breno R. C. Machado
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  3. Wilson G. de Morais Júnior
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  4. João R. M. Almeida
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  5. Sílvia B. Gonçalves
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Contributions

The manuscript was written through contributions of all authors.

Conceived and designed the experiments: TFP, BRCM

Analyzed the data: TFP, WGMJr, JRMA, SBG

Wrote the paper: TFP, WGMJr, JRMA, SBG

Corresponding author

Correspondence to Sílvia B. Gonçalves.

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Pacheco, T.F., Machado, B.R.C., de Morais Júnior, W.G. et al. Enhanced Tolerance of Spathaspora passalidarum to Sugarcane Bagasse Hydrolysate for Ethanol Production from Xylose. Appl Biochem Biotechnol 193, 2182–2197 (2021). https://doi.org/10.1007/s12010-021-03544-6

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