Abstract
This study examined the effects of different pretreatments of birch sawdust on the production and activity of polysaccharides by Inonotus obliquus, and in order to explore the mechanism, structural characterization and analysis were carried out. The result clearly indicated that alkali treatment, ozone treatment, and alkali combined with ozone treatment of birch sawdust could be all helpful for the production of active polysaccharide by I. obliquus. Among four pretreatment groups, birch sawdust treated with alkali showed the highest increase in the exo-polysaccharide content (39.90%) and the inhibition rate of α-glucosidase (80.78%) within 11 days by the mycelium of I. obliquus through deep fermentation, in comparison to water-washed birch sawdust. Through a single-factor analysis and orthogonal experimental design, the optimum alkali treatment condition was as follows: NaOH concentration 1%, temperature 60 °C, and time 3 h. Moreover, the structural characteristics of pretreated birch sawdust with the optimum alkali treatment condition before and after fermentation by the mycelium of I. obliquus was performed by Fourier transform infrared spectroscopy, X-ray diffraction, and scanning electronic microscopy. The results showed that alkali treatment destroyed the lignin structure of birch sawdust, exposed the cellulose in the amorphous area, reduced the crystallinity of lignocellulose, and damaged the surface structure of birch sawdust, which had a further damage and a greater degradation degree of birch sawdust after fermentation, indicating that alkali pretreatment was beneficial for utilization of birch sawdust by I. obliquus.
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References
Liu, P., Xue, J., Tong, S., Dong, W., & Wu, P. (2018). Structure characterization and hypoglycaemic activities of two polysaccharides from Inonotus obliquus. Molecules, 23(8), 1948.
Chen, H., Yan, M., Zhu, J., & Xu, X. (2011). Enhancement of exo-polysaccharide production and antioxidant activity in submerged cultures of Inonotus obliquus by lignocellulose decomposition. Journal of Industrial Microbiology & Biotechnology, 38(2), 291–298.
Xue, J., Tong, S., Wang, Z., & Liu, P. (2018). Chemical characterization and hypoglycaemic activities in vitro of two polysaccharides from Inonotus obliquus by Submerged Culture. Molecules, 23(12), 3261.
Ma, C.-Y., Wang, H.-M., Wen, J.-L., Shi, Q., Wang, S.-F., Yuan, T.-Q., & Sun, R.-C. (2020). Structural elucidation of lignin macromolecule from abaca during alkaline hydrogen peroxide delignification. International Journal of Biological Macromolecules, 144, 596–602.
Vanholme, R., De Meester, B., Ralph, J., & Boerjan, W. (2019). Lignin biosynthesis and its integration into metabolism. Current Opinion in Biotechnology, 56, 230–239.
Xiang, Y., Xu, X., & Li, J. (2012). Chemical properties and antioxidant activity of exopolysaccharides fractions from mycelial culture of Inonotus obliquus in a ground corn stover medium. Food Chemistry, 134(4), 1899–1905.
Syaftika, N., & Matsumura, Y. (2018). Comparative study of hydrothermal pretreatment for rice straw and its corresponding mixture of cellulose, xylan, and lignin. Bioresource Technology, 255, 1–6.
Kumar, A. K., & Sharma, S. (2017). Recent updates on different methods of pretreatment of lignocellulosic feedstocks: a review. Bioresources and Bioprocessing, 4(1), 7.
Kim, J. S., Lee, Y. Y., & Kim, T. H. (2016). A review on alkaline pretreatment technology for bioconversion of lignocellulosic biomass. Bioresource Technology, 199, 42–48.
Vidal, P. F., & Molinier, J. (1988). Ozonolysis of lignin-improvement of invitro digestibility of poplar sawdust. Biomass, 16(1), 1–17.
Lee, J. M., Jameel, H., & Venditti, R. A. (2010). Effect of ozone and autohydrolysis pretreatments on enzymatic digestibility of coastal Bermuda grass. Bioresources, 5, 1084–1101.
Wang, M., Zhao, Z., Zhou, X., Hu, J., Xue, J., Liu, X., Zhang, J., Liu, P. and Tong, S. (2019) Simultaneous use of stimulatory agents to enhance the production and hypoglycaemic activity of polysaccharides from Inonotus obliquus by Submerged Fermentation. Molecules, 24.
Xu, X.-q., Hu, Y., & Zhu, L.-h. (2014). The capability of Inonotus obliquus for lignocellulosic biomass degradation in peanut shell and for simultaneous production of bioactive polysaccharides and polyphenols in submerged fermentation. Journal of the Taiwan Institute of Chemical Engineers, 45, 2851–2858.
Xu, X., & Zhu, J. (2011). Enhanced phenolic antioxidants production in submerged cultures of Inonotus obliquus in a ground corn stover medium. Biochemical Engineering Journal, 58, 103–109.
Zhu, L., & Xu, X. (2013). Stimulatory effect of different lignocellulosic materials for phenolic compound production and antioxidant activity from Inonotus obliquus in submerged fermentation. Applied Biochemistry and Biotechnology, 169(7), 2138–2152.
Fang, S., Wang, W. H., Tong, S. S., Zhang, C. Y. and Liu, P. (2018) Evaluation of the effects of isolated lignin on cellulose enzymatic hydrolysis of corn stover pretreatment by NaOH combined with ozone. Molecules, 23.
DuBois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A., & Smith, F. (1956). Colorimetric method for determination of sugars and related substances. Analytical Chemistry, 28(3), 350–356.
Ren, Z., Qin, T., Qiu, F., Song, Y., Lin, D., Ma, Y., Li, J., & Huang, Y. (2017). Immunomodulatory effects of hydroxyethylated Hericium erinaceus polysaccharide on macrophages RAW264.7. International Journal of Biological Macromolecules, 105(Pt 1), 879–885.
Karimi, K., & Taherzadeh, M. J. (2016). A critical review of analytical methods in pretreatment of lignocelluloses: Composition, imaging, and crystallinity. Bioresource Technology, 200, 1008–1018.
Çetinkol, Ö. P., Dibble, D. C., Cheng, G., Kent, M. S., Knierim, B., Auer, M., Wemmer, D. E., Pelton, J. G., Melnichenko, Y. B., Ralph, J., Simmons, B. A., & Holmes, B. M. (2010). Understanding the impact of ionic liquid pretreatment on eucalyptus. Biofuels, 1(1), 33–46.
Moore, A. K., & Owen, N. L. (2001). Infrared spectroscopic studies of solid wood. Applied Spectroscopy Reviews, 36(1), 65–86.
Wang, B., Wang, X., & Feng, H. (2010). Deconstructing recalcitrant Miscanthus with alkaline peroxide and electrolyzed water. Bioresource Technology, 101(2), 752–760.
Kumar, R., Mago, G., Balan, V., & Wyman, C. E. (2009). Physical and chemical characterizations of corn stover and poplar solids resulting from leading pretreatment technologies. Bioresource Technology, 100(17), 3948–3962.
Li, C., Knierim, B., Manisseri, C., Arora, R., Scheller, H. V., Auer, M., Vogel, K. P., Simmons, B. A., & Singh, S. (2010). Comparison of dilute acid and ionic liquid pretreatment of switchgrass: Biomass recalcitrance, delignification and enzymatic saccharification. Bioresource Technology, 101(13), 4900–4906.
Yang, T., Yun, L., Hou, H., Xi, B., He, L., Wang, X., Huang, C., Wu, K., Zhao, Y., & Chen, B. (2014). Spatial structure characteristic analysis of corn stover during alkali and biological co-pretreatment using XRD. Bioresource Technology, 163, 356–359.
Shirkavand, E., Baroutian, S., Gapes, D. J., & Young, B. R. (2017). Pretreatment of radiata pine using two white rot fungal strains Stereum hirsutum and Trametes versicolor. Energy Conversion and Management, 142, 13–19.
Xu, X., Xu, Z., Shi, S., & Lin, M. (2017). Lignocellulose degradation patterns, structural changes, and enzyme secretion by Inonotus obliquus on straw biomass under submerged fermentation. Bioresource Technology, 241, 415–423.
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This study received financial support from the National Natural Science Foundation of China (21576282).
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All authors contributed to the study conception and design. Material preparation, data collection, and analysis were performed by Xiaohong Lu, Mengya Wang, and Zhezhen Zhao. The first draft of the manuscript was written by Xiaohong Lu. Ping Liu contributed to designing the experiments; Jinrong Hu and Jingsheng Zhang commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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Lu, X., Wang, M., Zhao, Z. et al. Effect of Different Pretreatment of Birch Sawdust on the Production of Active Polysaccharides by Inonotus obliquus Under Submerged Fermentation and Its Structural Mechanism. Appl Biochem Biotechnol 193, 1545–1557 (2021). https://doi.org/10.1007/s12010-021-03508-w
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DOI: https://doi.org/10.1007/s12010-021-03508-w