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Production of Short Chain Fructo-oligosaccharides from Inulin of Chicory Root Using Fungal Endoinulinase

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Abstract

Short chain fructo-oligosaccharides (SC-FOS) are the potential prebiotics possessing diverse applications in both food and feed industries. The present study was aimed to extract inulin from chicory roots followed by its conversion into SC-FOS applying endoinulinase from Aspergillus fumigatus. The inulin was extracted from chicory roots through boiling in hot water, followed by precipitation with ethanol at room temperature or freezing condition. Maximum yield (42%) of inulin was obtained with three volumes of chilled absolute ethanol at room temperature. HPLC analysis of enzymatic hydrolysate detected kestose (GF2), nystose (GF3), and other FOS having higher degree of polymerization (DP). Maximum GF2 (5.79 mg/ml) was detected at temperature 50 °C, pH 5.5 with 2 U of enzyme dose after 6 h of hydrolysis; while maximum GF3 (4.33 mg/ml) was recorded at 60 °C, 5.5 pH with 0.5 U enzyme dose after 2 h of hydrolysis. Nevertheless, complete hydrolysis of inulin was noticed with 99% total oligosaccharide yield at 55 °C, 5.5 pH with 0.5 U enzyme dose after 4 h of hydrolysis with negligible amount of mono- and di-saccharides. The present finding demonstrated the process for higher yield of inulin from chicory roots followed by its conversion into SC-FOS applying fungal endoinulinase.

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Acknowledgments

The authors gratefully acknowledge the Council of Scientific and Industrial Research, Ministry of Science and Technology, Government of India (File No. 09/1158(0002)2K17) for awarding the Senior Research Fellowship.

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Authors and Affiliations

  1. ICAR-National Institute of Animal Nutrition and Physiology, Adugodi, Hosur road, Bengaluru, Karnataka, 560030, India

    Jayaram Chikkerur, Ashis Kumar Samanta, Atul P. Kolte, Arindam Dhali & Sohini Roy

  2. Department of Microbiology, School of Sciences, Jain University, Bengaluru, Karnataka, 560011, India

    Jayaram Chikkerur & Sohini Roy

  3. SAARC Agriculture Centre, BARC Complex, Farmgate, Dhaka, 1215, Bangladesh

    Ashis Kumar Samanta

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  1. Jayaram Chikkerur
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Correspondence to Ashis Kumar Samanta.

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Chikkerur, J., Samanta, A.K., Kolte, A.P. et al. Production of Short Chain Fructo-oligosaccharides from Inulin of Chicory Root Using Fungal Endoinulinase. Appl Biochem Biotechnol 191, 695–715 (2020). https://doi.org/10.1007/s12010-019-03215-7

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