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Protein Production Through Microbial Conversion of Rice Straw by Multi-Strain Fermentation

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Abstract

Multi-strain mixed fermentation can provide a relatively complete lignocellulosic enzyme system compared with single-strain fermentation. This study was firstly to screen strains which have a strong ability to hydrolyse rice straw (RS) enzymatically and enrich true protein (TP). Then, the conditions in the process of SSF, including the optimum inoculum size of mixed strains, inoculation ratio, and different inoculation time of N. crassa 14–8, were optimized. The experimental results showed that the highest TP content could be obtained by using N. crassa 14–8, C. utilis, and P. chrysosporium as mixed strains, and 5 mM Mn2+ and 50 mM veratryl alcohol were used as inducers of lignin peroxidase (LiP) to improve the efficiency of enzymatic hydrolysis. When N. crassa 14–8 was inoculated 1 day later than P. chrysosporium, the total inoculum size was 10%, and the optimum ratio of N. crassa 14–8 to P. chrysosporium was 1:2, the maximum TP yield (8.89%) was obtained, with 123.37% of its increase rate. This work proposed a technique with potential application in large-scale feedstuff protein conversion.

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Acknowledgements

This work was supported by the Key R&D Program of Jiangsu Province (Modern Agriculture), China (BE2017355); the Agricultural Sci-Tech Self-Innovation Program of Jiangsu Province, CX(17)3044, China; the Open Funding Project of National Key Laboratory of Biochemical Engineering; and Jiangsu Special Research and Development Grant for Northern Jiangsu Area, China (SZ-YC2017001).

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Authors and Affiliations

  1. Institute of Life Sciences, Jiangsu University, Zhenjiang, 212000, P. R. China

    Jinru Jia, Huayou Chen, Bangguo Wu, Fengjie Cui, Hongcheng Wang & Zhong Ni

  2. Translational Research Institute, Henan Provincial People’s Hospital, Zhengzhou, 450000, P. R. China

    Jinru Jia

  3. National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, P. R. China

    Huayou Chen

  4. Jiangsu Yancheng Yuanyao Biotechnology Co., Ltd., Yancheng, 224000, P. R. China

    Fengjie Cui & Hua Fang

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  1. Jinru Jia
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Correspondence to Huayou Chen.

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Jia, J., Chen, H., Wu, B. et al. Protein Production Through Microbial Conversion of Rice Straw by Multi-Strain Fermentation. Appl Biochem Biotechnol 187, 253–265 (2019). https://doi.org/10.1007/s12010-018-2792-5

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  • DOI: https://doi.org/10.1007/s12010-018-2792-5

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