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Bioconversion of Welan Gum from Kitchen Waste by a Two-Step Enzymatic Hydrolysis Pretreatment

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Abstract

Kitchen waste (KW) is a worldwide issue, which can lead to environment pollution. Nevertheless, it is also a low-cost and sustainable resource for bio-production. Meanwhile, welan gum (WG) is one kind of the most important exopolysaccharide but with high material cost. The aim of this study was to adopt two-step enzymatic hydrolysis to improve the release and recovery of both sugar and protein in KW for subsequent WG production. As the results, the recovery rates of sugar and protein reached 81.07 and 77.38%, which were both satisfactory. After the conditions optimized in flasks, the welan fermentation was conducted in a 5-L fermentor, and the WG yield, utilization rates of reducing sugar and KDN, respectively, reached 5.57 g L−1, 94.25% and 61.96%. Moreover, the kinetic analyses demonstrated that the WG fermentation in KWH was a partly growth-associated process. The KW was successfully treated by fermentation for the bioconversion to WG.

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Abbreviations

KW:

Kitchen waste

KWH:

Kitchen waste hydrolysate

WG:

Welan gum

KDN:

Kjeldahl nitrogen

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Acknowledgements

This work was supported by Basic Application Program of Department of Science and Technology of Sichuan Province (Grant No. 2015JY0241).

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Authors and Affiliations

  1. Department of Pharmaceutical and Biological Engineering, School of Chemical Engineering, Sichuan University, Chengdu, Sichuan, 610065, China

    Panyu Li, Yi Xie, Yu Zeng, Wanrong Hu, Yan Kang, Xiang Li, Yabo Wang, Tonghui Xie & Yongkui Zhang

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  1. Panyu Li
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Correspondence to Yongkui Zhang.

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Li, P., Xie, Y., Zeng, Y. et al. Bioconversion of Welan Gum from Kitchen Waste by a Two-Step Enzymatic Hydrolysis Pretreatment. Appl Biochem Biotechnol 183, 820–832 (2017). https://doi.org/10.1007/s12010-017-2466-8

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  • DOI: https://doi.org/10.1007/s12010-017-2466-8

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