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Enhancing Menaquinone-7 Production by Bacillus natto R127 Through the Nutritional Factors and Surfactant

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Abstract

Bacillus natto is commonly used in industrial production of menaquinone-7, an important vitamin which plays a crucial role for blood clotting and may contribute to prevention of cardiovascular disease and osteoporosis. This study determined the optimal combination of key nutrients and established an effective use of surfactant in a coupling medium to enhance the yield of extracellular MK-7. MK-7 yield of 31.18 mg/L was achieved under optimal conditions containing 53.6 g/L glycerol, 100 g/L soy peptone, and 10 g/L K2HPO4. A maximal yield of 40.96 mg/L MK-7 and a secretion ratio of 61.1% were obtained when 20 g/L soybean oil was supplemented at the logarithmic phase. The non-ionic surfactant span 20 was the second most promising surfactant in improving product yield, whereas addition of 2 g/L betaine exerted a minimal effect on secretion ratio of MK-7 at 19.1%. The results collectively showed that the supplementation of surfactants was an effective strategy to regulate cytomembrane permeability.

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    Authors and Affiliations

    1. Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), College of Biotechnology and Pharmaceutical Engineering, School of Pharmacy, Nanjing Tech University, No. 30 South Puzhu Road, Nanjing, 211816, People’s Republic of China

      Xue-chao Hu, Miao-miao Luo, Lu-jing Ren, Xiao-jun Ji & He Huang

    2. Bioengineering Institute, Department of Chemical and Biological Engineering, Zhejiang University, No. 38 Zheda Road, Hangzhou, 310027, People’s Republic of China

      Wei-ming Liu

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    1. Xue-chao Hu
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    2. Wei-ming Liu
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    3. Miao-miao Luo
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    Correspondence to Lu-jing Ren or He Huang.

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    This work was financially supported by the National Science Foundation for Distinguished Young Scholars of China (No. 21225626), the National Natural Science Foundation of China (No. 21306085 and No. 21476111), Jiangsu Province Outstanding Youth Fund (BK20160092), the National High Technology Research and Development Program of China (No.2014AA021701), the Specialized Research Fund for the Doctoral Program of Higher Education (No. 20133221120008), and the selected project of Nanjing Tech University (No. ZKRC201510).

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    Xue-chao Hu and Wei-ming Liu contributed equally as co-first author.

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    Hu, Xc., Liu, Wm., Luo, Mm. et al. Enhancing Menaquinone-7 Production by Bacillus natto R127 Through the Nutritional Factors and Surfactant. Appl Biochem Biotechnol 182, 1630–1641 (2017). https://doi.org/10.1007/s12010-017-2423-6

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