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Effects of Chromosomal Integration of the Vitreoscilla Hemoglobin Gene (vgb) and S-Adenosylmethionine Synthetase Gene (metK) on ε-Poly-l-Lysine Synthesis in Streptomyces albulus NK660

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Abstract

ε-Poly-l-lysine (ε-PL) is a widely used natural food preservative. To test the effects of the Vitreoscilla hemoglobin (VHb) and S-adenosylmethionine (SAM) on ε-PL synthesis in Streptomyces albulus NK660, the heterologous VHb gene (vgb) and SAM synthetase gene (metK) were inserted into the S. albulus NK660 chromosome under the control of the constitutive ermE* promoter. CO-difference spectrum analysis showed S. albulus NK660-VHb strain could express functional VHb. S. albulus NK660-VHb produced 26.67 % higher ε-PL and 14.57 % higher biomass than the wild-type control, respectively. Reversed-phase high-pressure liquid chromatography (RP-HPLC) results showed the overexpression of the metK gene resulted in increased intracellular SAM synthesis in S. albulus NK660-SAM, which caused increases of biomass as well as the transcription level of ε-PL synthetase gene (pls). Results indicated that the expression of vgb and metK gene improved on ε-PL synthesis and biomass for S. albulus NK660, respectively.

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Acknowledgments

This work was supported by National key Basic Research Program of China (“973”-Program) 2012CB725204, National Key Technology R&D Program No. 2015BAD16B04, Natural Science Foundation of China Grant Nos. 31470213 and 31300032, Project of Tianjin, China (13JCZDJC27800, 13TXSYJC40100 and 14ZCZDSF00009).

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    Authors and Affiliations

    1. Key Laboratory of Molecular Microbiology and Technology for Ministry of Education, Nankai University, Tianjin, 300071, China

      Yanyan Gu, Xiaomeng Wang, Chao Yang, Weitao Geng, Jun Feng, Yuanyuan Wang & Cunjiang Song

    2. State Key Laboratory of Medicinal Chemical Biology, Nankai University, 94 Weijin Road, Tianjin, 300071, China

      Shufang Wang

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    Correspondence to Shufang Wang or Cunjiang Song.

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    Yanyan Gu and Xiaomeng Wang contributed equally to this work.

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    Gu, Y., Wang, X., Yang, C. et al. Effects of Chromosomal Integration of the Vitreoscilla Hemoglobin Gene (vgb) and S-Adenosylmethionine Synthetase Gene (metK) on ε-Poly-l-Lysine Synthesis in Streptomyces albulus NK660. Appl Biochem Biotechnol 178, 1445–1457 (2016). https://doi.org/10.1007/s12010-015-1958-7

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