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Comparative Study of Biosafety, DNA, and Chromosome Damage of Different-Materials-Modified Fe3O4 in Rats

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Abstract

The increasing use of modified Fe3O4 magnetic microparticles has raised safety concerns regarding their use and effect on human health. This study assessed the in vivo biosafety, DNA, and chromosome damage of modified Fe3O4 microparticles such as Au@Fe3O4, Ag@Fe3O4, Cs@Fe3O4, Pt@Fe3O4, and CdS@Fe3O4, using spleen-deficient rats. Spleen-deficient rats treated with naked and modified (Au, Cs, Pt) Fe3O4 microparticles (5000 mg/kg) displayed low toxicity. Only treatment with Cds@Fe3O4 resulted in elevated toxicity and death in rats. Au-, Ag-, and Pt-modified Fe3O4 increased the rate of hemolysis in rats relative to treatment with naked Fe3O4. Despite this, Au- and Pt-modified Fe3O4 increased the biocompatibility and reduced DNA and chromosome damage in rats relative to naked Fe3O4. While Cs@Fe3O4 microparticles displayed a higher biocompatibility than naked Fe3O4, they displayed no significant reduction in DNA and chromosome damage. In summary, Au and Pt surface-modified Fe3O4 microparticles display elevated in vivo biosafety compared to unmodified particles. The precious metal material, with good biological compatibility, surface modification of Fe3O4 is an effective strategy to improve the overall safety and potential therapeutic utility of these magnetic materials.

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Acknowledgments

This work was financially supported by the National Natural Science Foundation of China (21175075), the Natural Science Foundation of Jiangsu Province (BK2012651, BK2012652, BK2011047), the Application Research Item of Nantong City (BK2013028), the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD), and the “Dr. Offer plan” of Jiangsu province.

Conflict of interest

The authors declare that they have no competing interests.

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Authors and Affiliations

  1. School of Public Health, Nantong University, Nantong, Jiangsu, 226019, China

    Dong-Lin Xia, Yan-Pei Chen, Chao Chen, Yu-Fei Wang, Xiao-dong Li & Hai-Ying Gu

  2. Affiliated Hospital of Nantong University, Nantong, Jiangsu, 226001, China

    Hong He

  3. Institute of Analytical Chemistry for Life Science, Nantong University, Nantong, Jiangsu, 226019, China

    Dong-Lin Xia, Chao Chen, Yu-Fei Wang & Hai-Ying Gu

  4. Nantong Tongda Chemicals Safety Evaluation Center Co. Ltd., Nantong, Jiangsu, 226019, China

    Xiao-dong Li, Hong He & Hai-Ying Gu

  5. Nantong University of Xinglin College, Nantong, Jiangsu, 226001, People’s Republic of China

    Hai-Ying Gu

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  1. Dong-Lin Xia
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Correspondence to Hai-Ying Gu.

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Dong-Lin Xia and Yan-Pei Chen equally contributed to this work.

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Xia, DL., Chen, YP., Chen, C. et al. Comparative Study of Biosafety, DNA, and Chromosome Damage of Different-Materials-Modified Fe3O4 in Rats. Appl Biochem Biotechnol 177, 1069–1082 (2015). https://doi.org/10.1007/s12010-015-1797-6

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  • DOI: https://doi.org/10.1007/s12010-015-1797-6

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