Abstract
Adenosine is one of the nucleoside which plays an important role in signal transduction and neuromodulation. This work proposes a simple electrochemical assay, comprising two enzymes and rhodium complex based electron transfer mediator, for the detection of adenosine. Sequential reaction of adenosine deaminase and l-glutamic dehydrogenase and the supporting cycle between β-NADH and mediator enable quantitative analysis of adenosine. Role of electron transfer mediator is the conveyance of proton from electrode to β-NAD+ for regeneration of β-NADH. The electrochemical characteristics of electron transfer mediator were also studied. Real-time adenosine detection was carried out using this multiple enzyme based chronoamperometric assay. The analysis results show a low limit of detection (140 μM) and good correspondence between current signal and the adenosine concentration (R 2 = 0.997).
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This work was supported by Business for Cooperative R&D between Industry, Academy, and Research Institute funded by Korea Small and Medium Business Administration in 2014 (grant No. C0219103).
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Han, D., Kim, HM., Chand, R. et al. Rhodium Complex and Enzyme Couple Mediated Electrochemical Detection of Adenosine. Appl Biochem Biotechnol 177, 812–820 (2015). https://doi.org/10.1007/s12010-015-1779-8
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DOI: https://doi.org/10.1007/s12010-015-1779-8