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Rhodium Complex and Enzyme Couple Mediated Electrochemical Detection of Adenosine

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Abstract

Adenosine is one of the nucleoside which plays an important role in signal transduction and neuromodulation. This work proposes a simple electrochemical assay, comprising two enzymes and rhodium complex based electron transfer mediator, for the detection of adenosine. Sequential reaction of adenosine deaminase and l-glutamic dehydrogenase and the supporting cycle between β-NADH and mediator enable quantitative analysis of adenosine. Role of electron transfer mediator is the conveyance of proton from electrode to β-NAD+ for regeneration of β-NADH. The electrochemical characteristics of electron transfer mediator were also studied. Real-time adenosine detection was carried out using this multiple enzyme based chronoamperometric assay. The analysis results show a low limit of detection (140 μM) and good correspondence between current signal and the adenosine concentration (R 2 = 0.997).

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References

  1. Philis, J. W. (1989). Adenosine in the control of the cerebral circulation. Cerebrovascular and Brain Metabolism Reviews, 1, 26–54.

    Google Scholar 

  2. Latini, S., & Pedata, F. (2008). Adenosine in the central nervous system: release mechanisms and extracellular concentrations. Journal of Neurochemistry, 79, 463–484.

    Article  Google Scholar 

  3. Perry, M., Li, Q., & Kennedy, R. T. (2009). Review of recent advances in analytical techniques for the determination of neurotransmitters. Analytica Chimica Acta, 653, 1–22.

    Article  CAS  Google Scholar 

  4. Li, F., Zhang, J., Cao, X., Wang, L., Li, D., Song, S., Ye, B., & Fan, C. (2009). Adenosine detection by using gold nanoparticles and designed aptamer sequences. The Analyst, 134, 1355.

    Article  CAS  Google Scholar 

  5. Cui, Y., Barford, J. P., & Renneberg, R. (2008). Amperometric trienzyme ATP biosensors based on the coimmobilization of salicylate hydroxylase, glucose-6-phosphate dehydrogenase, and hexokinase. Sensors and Actuators B: Chemical, 132, 1–4.

    Article  CAS  Google Scholar 

  6. Swamy, B. E. K., & Venton, B. J. (2007). Subsecond detection of physiological adenosine concentrations using fast-scan cyclic voltammetry. Analytical Chemistry, 79, 744–750.

    Article  CAS  Google Scholar 

  7. Yan, F., Wang, F., & Chen, Z. (2011). Aptamer-based electrochemical biosensor for label-free voltammetric detection of thrombin and adenosine. Sensors and Actuators B: Chemical, 160, 1380–1385.

    Article  CAS  Google Scholar 

  8. Zhang, M., Karra, S., & Gorski, W. (2013). Rapid electrochemical enzyme assay with enzyme-free calibration. Analytical Chemistry, 85, 6026–6032.

    Article  CAS  Google Scholar 

  9. Bartling, B., Li, L., & Liu, C.-C. (2010). Detection of adenosine deaminase activity with a thick-film screen-printed Ir/C sensor to detect liver disease. Journal of The Electrochemical Society, 157, J130.

    Article  CAS  Google Scholar 

  10. Hollmann, F., Witholt, B., & Schmid, A. (2002). [Cp∗Rh(bpy)(H2O)]2+: a versatile tool for efficient and non-enzymatic regeneration of nicotinamide and flavin coenzymes. Journal of Molecular Catalysis B: Enzymatic, 19–20, 167–176.

    Article  Google Scholar 

  11. Du, Y., Hyster, T. K., & Rovis, T. (2011). Rhodium(iii)-catalyzed oxidative carbonylation of benzamides with carbon monoxide. Chemical Communications, 47, 12074.

    Article  CAS  Google Scholar 

  12. Grammenudi, S., Franke, M., Vögtle, F., & Steckhan, E. (1987). The rhodium complex of a tris(bipyridine) ligand? Its electrochemical behaviour and function as mediator for the regeneration of NADH from NAD+. Journal of Inclusion Phenomena, 5, 695–707.

    Article  CAS  Google Scholar 

  13. Steckhan, E., Herrmann, S., Ruppert, R., Thömmes, J., & Wandrey, C. (1990). Continuous generation of NADH from NAD⊕ and formate using a homogeneous catalyst with enhanced molecular weight in a membrane reactor. Angewandte Chemie International Edition in English, 29, 388–390.

    Article  Google Scholar 

  14. Song, H.-K., Lee, S. H., Won, K., Park, J. H., Kim, J. K., Lee, H., Moon, S.-J., Kim, D. K., & Park, C. B. (2008). Electrochemical regeneration of NADH enhanced by platinum nanoparticles. Angewandte Chemie International Edition, 47, 1749–1752.

    Article  CAS  Google Scholar 

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Acknowledgments

This work was supported by Business for Cooperative R&D between Industry, Academy, and Research Institute funded by Korea Small and Medium Business Administration in 2014 (grant No. C0219103).

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Correspondence to Ik-Soo Shin or Yong-Sang Kim.

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Han, D., Kim, HM., Chand, R. et al. Rhodium Complex and Enzyme Couple Mediated Electrochemical Detection of Adenosine. Appl Biochem Biotechnol 177, 812–820 (2015). https://doi.org/10.1007/s12010-015-1779-8

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  • DOI: https://doi.org/10.1007/s12010-015-1779-8

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