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Immobilizing Yarrowia lipolytica Lipase Lip2 via Improvement of Microspheres by Gelatin Modification

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Abstract

The purpose of this study was to investigate the feasibility of immobilizing Yarrowia lipolytica lipase lip2 on epoxy microspheres with or without gelatin modifications. The activity of lipase immobilized on gelatin-modified supports was twofold higher than those immobilized on native supports. There was no significant difference in the Michaelis-Menten constant (K M ) between the two immobilized lipases. However, lipase immobilized on gelatin modified supports showed an approximately fourfold higher V max than lipase immobilized on native supports. Lipase immobilization on the gelatin-modified support exhibited a significantly improved operational stability in an esterification system. After it was reused for a total of 35 batches, the ester conversion of lipase immobilized on gelatin-modified and native microspheres was 83 and 60 %, respectively. Furthermore, the immobilized lipase could be stored at 4 °C for 12 months without any loss of activity.

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Acknowledgments

This work was supported by the National Basic Research Program of China (973 program) (2013CB733600, 2012CB725200), the National Nature Science Foundation of China (21106005), and National High-Tech R&D Program of China (863 Program) (2012AA022205D).

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Authors and Affiliations

  1. National Energy R&D Center for Biorefinery, Beijing Key Laboratory of Bioprocess, College of Biology Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, People’s Republic of China

    Rong Xie, Caixia Cui, Biqiang Chen & Tianwei Tan

Authors
  1. Rong Xie
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  2. Caixia Cui
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  3. Biqiang Chen
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  4. Tianwei Tan
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Corresponding author

Correspondence to Biqiang Chen.

Additional information

Rong Xie and Caixia Cui are equal contributors.

Research Highlights

• Novel macroporous beads were used as the matrix for immobilization.

• A simple, efficient immobilization protocol on microsphere beads was developed.

• Gelatin was used to regulate the micro-environment.

• The activity of lipase immobilized on gelatin-support was improved two times.

• Lipase immobilized on gelatin-support could be reused 35 batches for esterification.

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Xie, R., Cui, C., Chen, B. et al. Immobilizing Yarrowia lipolytica Lipase Lip2 via Improvement of Microspheres by Gelatin Modification. Appl Biochem Biotechnol 177, 771–779 (2015). https://doi.org/10.1007/s12010-015-1771-3

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  • DOI: https://doi.org/10.1007/s12010-015-1771-3

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