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Effect of Ethanol Accumulation on Porcine Interferon-α Production by Pichia pastoris and Activities of Key Enzymes in Carbon Metabolism

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Abstract

In production of porcine interferon α (pIFN-α) by Pichia pastoris, improper glycerol feeding strategy leads to ethanol accumulation in the last stage of growth phase. In the present study, taking two runs with low ethanol accumulation under 2 g/L as control group, effects of long-term (>4 h) and instantaneous high ethanol concentration (>10 g/L) on pIFN-α production, and activities of key enzymes in carbon metabolism were discussed. As a result, compared with control group, pIFN-α expression level was decreased about 4~12 folds under long-term high ethanol concentration, from the level above 3 g/L to the level under 1 g/L; pIFN-α expression level was decreased about 8 folds under instantaneous high ethanol concentration, reaching to the low level of 0.42 g/L. The low production of pIFN-α was caused by the severe inhibitory effect of ethanol on these enzymes.

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Acknowledgments

The authors thank the financial supports from the Natural Science Foundation of China (#31301408), Natural Science Foundation of Jiangsu Province (#BK20130122), and the 111 Project (No. 111–2–06).

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Authors and Affiliations

  1. The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 214122, Wuxi, China

    Jian Ding, Minjie Gao, Guoli Hou & Kexue Liang

  2. School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi, 214122, China

    Jian Ding

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  1. Jian Ding
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  2. Minjie Gao
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Correspondence to Jian Ding.

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Ding, J., Gao, M., Hou, G. et al. Effect of Ethanol Accumulation on Porcine Interferon-α Production by Pichia pastoris and Activities of Key Enzymes in Carbon Metabolism. Appl Biochem Biotechnol 176, 1964–1974 (2015). https://doi.org/10.1007/s12010-015-1693-0

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  • DOI: https://doi.org/10.1007/s12010-015-1693-0

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