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An Organophosphorus Hydrolase-Based Biosensor for Direct Detection of Paraoxon Using Silica-Coated Magnetic Nanoparticles

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Abstract

Rapid detection of organophosphorous (OP) compounds such as paraoxon would allow taking immediate decision on efficient decontamination procedures and could prevent further damage and potential casualties. In the present study, a biosensor based on nanomagnet-silica core-shell conjugated to organophosphorous hydrolase (OPH) enzyme was designed for detection of paraoxon. Coumarin1, a competitive inhibitor of the OPH enzyme, was used as a fluorescence-generating molecule. Upon excitation of cumarin1 located at the active site of the enzyme, i.e., OPH, the emitted radiations were intensified due to the mirroring effect of the nanomagnet-silica core-shell conjugated to the enzyme. In presence of paraoxon and consequent competition with the fluorophore in occupying enzyme’s active site, a significant reduction in emitted radiations was observed. This reduction was proportional to paraoxon concentration in the sample. The method worked in the 10- to 250-nM concentration range had a low standard deviation (with a coefficient of variation (CV) of 6–10 %), and the detection limit was as low as 5 × 10−6 μM.

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Acknowledgments

The authors would like to thank Nanozino Co. and Nanosystems Research Team (NRTeam) for financially supporting the present research.

Compliance with Ethical Standards

The authors declare no conflict of interest. No ethical approval was required.

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Authors and Affiliations

  1. Department of Toxicology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

    Reza Khaksarinejad & Rezvan Karami

  2. Research & Development Department, Nanozino Co., Tehran, Iran

    Afshin Mohsenifar & Tavoos Rahmani-Cherati

  3. Nanobiosensor Research Group, Nanosystems Research Team (NRTeam), Karaj, Iran

    Afshin Mohsenifar & Meisam Tabatabaei

  4. Microbial Biotechnology and Biosafety Department, Agricultural Biotechnology Research Institute of Iran (ABRII), AREEO, Karaj, Iran

    Meisam Tabatabaei

Authors
  1. Reza Khaksarinejad
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  2. Afshin Mohsenifar
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  3. Tavoos Rahmani-Cherati
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  4. Rezvan Karami
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  5. Meisam Tabatabaei
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Correspondence to Afshin Mohsenifar or Meisam Tabatabaei.

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Khaksarinejad, R., Mohsenifar, A., Rahmani-Cherati, T. et al. An Organophosphorus Hydrolase-Based Biosensor for Direct Detection of Paraoxon Using Silica-Coated Magnetic Nanoparticles. Appl Biochem Biotechnol 176, 359–371 (2015). https://doi.org/10.1007/s12010-015-1579-1

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  • DOI: https://doi.org/10.1007/s12010-015-1579-1

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