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Increased Vulnerability to Physical Stress by Inactivation of NdgR in Streptomyces coelicolor

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Abstract

The antibiotic production and spore formation process in Streptomyces coelicolor need complex decision making processes by several regulatory units. These regulatory units are involved in both primary and secondary metabolism. As a result, most regulators have several functions, and those are worthwhile themes to study about different functions of a known regulator. In this study, a deletion mutant of ndgR, which encodes the nitrogen-dependent growth regulator, was examined by the cell viability test, TEM, and growth in N-acetylglucosamine/asparagine (GlcNAc/Asn) liquid medium. The results of the study show that NdgR is also involved in the structure of the cell membrane affecting survival under physical shocks. Deletion of ndgR leads to abnormal cell membrane resulting in the vulnerable cells to physical stress caused by shaking with beads in liquid culture condition. This empirical observation is the first meaningful explanation to why ndgR mutant could not grow well in a liquid minimal medium due to the defect of N-acetylglucosamine (GlcNAc) utilization and phospholipid synthesis.

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Acknowledgments

The study was partially supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2013R1A1A2A10004690), by KOPRI (PE14030), by the R&D Program of MOTIE/KEIT (10049674) and the Energy Efficiency & Resources of the Korea Institute of Energy Technology Evaluation and Planning (KETEP) grant funded by the Korea Government Ministry of Trade, Industry and Energy (20133030000300).

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    Authors and Affiliations

    1. School of Chemical and Biological Engineering, Seoul National University, Kwanak-Gu, 151-742, Seoul, Korea

      Bo-Rahm Lee, Eunjung Song, Sung-Hee Park & Byung-Gee Kim

    2. Department of Microbial Engineering, College of Engineering, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul, 143-701, Korea

      Da-Hye Yi, Shashi Kant Bhatia, Ju Hee Lee & Yung-Hun Yang

    3. Chemical Engineering, Soongsil University, 511 Sangdo-dong, Seoul, 156-743, Republic of Korea

      Yun-Gon Kim

    4. Division of Life Sciences, Korea Polar Research Institute, 12 Gaetbeol-ro, Yeonsu-gu, Incheon, 406-840, Korea

      Yoo Kyung Lee

    5. Institute for Ubiquitous Information Technology and Applications (CBRU), Konkuk University, Seoul, 143-701, South Korea

      Yung-Hun Yang

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    1. Bo-Rahm Lee
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    Correspondence to Yung-Hun Yang.

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    Bo-Rahm Lee and Da-Hye Yi contributed equally to this work.

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    Lee, BR., Yi, DH., Song, E. et al. Increased Vulnerability to Physical Stress by Inactivation of NdgR in Streptomyces coelicolor . Appl Biochem Biotechnol 175, 3673–3682 (2015). https://doi.org/10.1007/s12010-015-1536-z

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    • DOI: https://doi.org/10.1007/s12010-015-1536-z

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