Abstract
Light oil from pyrolysis, which accounts for ∼10 % carbon yield of the starting biomass, is a complex aqueous product that is difficult to utilize and usually discarded. This work presents the feasibility of light oil as a sole carbon source to support the growth of Rhodococcus opacus (R. opacus) that in turn accumulate triacylglycerols as biodiesel feedstock. Two types of bacteria (R. opacus PD630 and DSM 1069) were selected in this study. Research results showed that after short adaption periods both strains can grow well on this complex carbon source, as proved by the consumption of oligomers and monomers in light oil. Lipid content by R. opacus PD630 and DSM 1069 was observed up to 25.8 % and 22.0 % of cell dry weight, respectively. Palmitic and stearic acids were found to be the predominant fatty acids in these bacterial cells. In addition, the light oil-based lipid production can be enhanced by reducing the pH value from 7 to 4, especially in case of DSM 1069.
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Acknowledgments
Z. Wei is grateful to China Scholarship Council for awarding a scholarship under the State Scholarship Fund to pursue her study. This work will be used by Z. Wei for partial fulfillment of the degree requirement for her doctoral research at the College of Environmental Science and Engineering, Hunan University, Changsha, China. We also wish to acknowledge DOE (EE0006112) for their support via Synthetic Design of Microorganisms for Lignin Fuel.
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Wei, Z., Zeng, G., Kosa, M. et al. Pyrolysis Oil-Based Lipid Production as Biodiesel Feedstock by Rhodococcus opacus . Appl Biochem Biotechnol 175, 1234–1246 (2015). https://doi.org/10.1007/s12010-014-1305-4
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DOI: https://doi.org/10.1007/s12010-014-1305-4