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Vacuum Stripping of Ethanol during High Solids Fermentation of Corn

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Abstract

In corn-ethanol industry, yeast stress inducing glucose concentrations produced during liquefaction and subsequent high ethanol concentrations produced during fermentation restrict slurry solids to 32 % w/w. These limits were circumvented by combining two novel technologies: (1) granular starch hydrolyzing enzyme (GSHE) to break down starch simultaneously with fermentation and (2) vacuum stripping to remove ethanol. A vacuum stripping system was constructed and applied to fermentations at 30, 40, and 45 % solids. As solids increased from 30 to 40 %, ethanol yield decreased from 0.35 to 0.29 L/kg. Ethanol yield from 45 % solids was only 0.18 L/kg. An improvement was conducted by increasing enzyme dose from 0.25 to 0.75 g/g corn and reducing yeast inoculum by half. After improvement, ethanol yield from 40 % solids vacuum treatment increased to 0.36 L/kg, comparable to ethanol yield from 30 % solids (control).

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References

  1. EISA. (2007). Energy Independence and Security Act. Technical Report Pub. L. 110-140. Washington DC: U.S. Government Printing Office.

    Google Scholar 

  2. Thatipamala, R., Rohani, S., & Hill, G. A. (1992). Biotechnology and Bioengineering, 40, 289–297.

    Article  CAS  Google Scholar 

  3. Wang, S., Ingledew, W. M., Thomas, K. C., Sosulski, K., & Sosulski, F. W. (1999). Cereal Chemistry, 76, 82–86.

    Article  CAS  Google Scholar 

  4. Ramalingham, A., & Finn, R. K. (1977). Biotechnology and Bioengineering, 19, 583–589.

    Article  CAS  Google Scholar 

  5. Cysewski, G. R., & Wilke, C. R. (1977). Biotechnology and Bioengineering, 19, 1125–1143.

    Article  CAS  Google Scholar 

  6. Taylor, F., Kurantz, M. J., Goldberg, N., McAloon, A. J., & Craig, J. C., Jr. (2000). Biotechnology Progress, 16, 541–547.

    Article  CAS  Google Scholar 

  7. Shihadeh, J., Huang, H., Raush, K. D., Tumbleson, E. T., & Singh, V. (2013). Transactions of the ASABE, 56, 1441–1447.

    CAS  Google Scholar 

  8. AACC International. (2002). Approved methods of the American Association of Cereal Chemists (Method 44-19 10th ed.). St. Paul: The Association.

    Google Scholar 

  9. Genencor International. (2005). Stargen 001, granular starch hydrolyzing enzyme for ethanol production. Palo Alto: Genencor International.

    Google Scholar 

  10. Casey, G. P., & Ingledew, W. M. (1986). CRC Critical Reviews in Microbiology, 13, 219–280.

    Article  CAS  Google Scholar 

  11. Singh, V., Johnson, D. B., Naidu, K., Rausch, K. D., Belyea, R. L., & Tumbleson, M. E. (2005). Cereal Chemistry, 82, 187–190.

    Article  CAS  Google Scholar 

  12. Ebell, L. F. (1969). Phytochemistry, 8, 25–36.

    Article  CAS  Google Scholar 

  13. Vidal, C. B., Rausch, K. D., Tumbleson, M. E., & Singh, V. (2009). Cereal Chemistry, 86, 133–135.

    Article  CAS  Google Scholar 

  14. Huang, H., Liu, W., Singh, V., Danao, M. G. C., & Eckhoff, S. R. (2012). Cereal Chemistry, 89, 217–221.

    Article  CAS  Google Scholar 

  15. Roychoudhury, P. K., Chose, T. K., Ghosh, P., & Chotani, G. K. (1986). Biotechnology and Bioengineering, 28, 972–976.

    Article  CAS  Google Scholar 

  16. Wang, P., Singh, V., Xue, H., Johnston, D. B., Rausch, K. D., & Tumbleson, M. E. (2007). Cereal Chemistry, 84, 10–14.

    Article  CAS  Google Scholar 

  17. Maiorella, B. L., Blanch, H. W., & Wilke, C. R. (1983). Biotechnology and Bioengineering, 25, 103–121.

    Article  CAS  Google Scholar 

  18. Russell, I. (2003). Chapter 9: Understanding Saccharomyces yeast fundamentals. In K. A. Jacques, T. P. Lyons, & D. R. Kelsall (Eds.), The alcohol textbook: a reference for the beverage, fuel and industrial alcohol industries (4th ed., pp. 103–110). Nottingham: Nottingham University Press.

    Google Scholar 

  19. Narendranath, N. V., Thomas, K. C., & Ingledew, W. M. (2001). Journal of Industrial Microbiology and Biotechnology, 26, 171–177.

    Article  CAS  Google Scholar 

  20. Kelsall, D. R., & Lyons, T. P. (2003). Chapter 10: Practical management of yeast: Conversion of sugars to ethanol. In K. A. Jacques, T. P. Lyons, & D. R. Kelsall (Eds.), The alcohol textbook: a reference for the beverage, fuel and industrial alcohol industries (4th ed., pp. 121–133). Nottingham: Nottingham University Press.

    Google Scholar 

  21. Taylor, F., Kurantz, M. J., Goldberg, N., McAloon, A. J., & Craig, J. C. (2000). Biotechnology Progress, 16, 541–547.

    Article  CAS  Google Scholar 

  22. Maiorella, B. L., Blanch, H. W., & Wilke, C. R. (1984). Biotechnology and Bioengineering, 26, 1155–1166.

    Article  CAS  Google Scholar 

  23. Singh, V., Panchal, C. B., & Eckhoff, S. R. (1999). Cereal Chemistry, 76, 846–849.

    Article  CAS  Google Scholar 

  24. Wilkins, M. R., Belyea, R. L., Singh, V., Buriak, P., Wallig, M. A., Tumbleson, M. E., & Rausch, K. D. (2006). Cereal Chemistry, 83, 121–126.

    Article  CAS  Google Scholar 

  25. Crabtree, H. G. (1929). Biochemical Journal, 23, 536–545.

    CAS  Google Scholar 

  26. Deken, R. H. D. (1966). Journal of General Microbiology, 44, 149–156.

    Article  Google Scholar 

  27. Maiorella, B. L., Wilke, C. R., & Blanch, H. W. (1981). Advances in Biochemical Engineering/Biotechnology, 20, 43–92.

    Article  CAS  Google Scholar 

  28. Lewis, S. M. (2007). Method for producing ethanol using raw starch. U.S. Patent Appl. No. 2007/0196907A1.

  29. Kelsall, D. R., & Lyons, T. P. (2003). Chapter 10: Grain dry milling and cooking procedures: Extracting sugars in preparation for fermentation. In K. A. Jacques, T. P. Lyons, & D. R. Kelsall (Eds.), The alcohol textbook: a reference for the beverage, fuel and industrial alcohol industries (4th ed., pp. 9–21). Nottingham: Nottingham University Press.

    Google Scholar 

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Acknowledgments

This project was supported by the USDA National Institute of Food and Agriculture, Hatch project No ILLU-741-333.

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Authors and Affiliations

  1. Agricultural and Biological Engineering, University of Illinois at Urbana-Champaign, 360G AESB, 1304 W. Pennsylvania Ave., Urbana, IL, 61802, USA

    Jameel K. Shihadeh, Haibo Huang, Kent D. Rausch, Mike E. Tumbleson & Vijay Singh

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  1. Jameel K. Shihadeh
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  2. Haibo Huang
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  3. Kent D. Rausch
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  4. Mike E. Tumbleson
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  5. Vijay Singh
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Correspondence to Vijay Singh.

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Mention of trade names or commercial products is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the University of Illinois at Urbana-Champaign.

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Shihadeh, J.K., Huang, H., Rausch, K.D. et al. Vacuum Stripping of Ethanol during High Solids Fermentation of Corn. Appl Biochem Biotechnol 173, 486–500 (2014). https://doi.org/10.1007/s12010-014-0855-9

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