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Regional Differences in Rice Hulls Supply for Bioethanol Production

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Abstract

Agricultural by-products are becoming an attractive substrate for bioethanol production. The aim of this study was to evaluate the effects of regional differences in the rice hulls using Escherichia coli KO11 for bioethanol production. The rice hulls coded Edirne were obtained from Thrace Region, and the rice hulls coded Izmir were obtained from Aegean Region in Turkey. Rice hulls were treated by dilute acid before using them as substrates. The cells were incubated on an orbital shaker at 160 rpm under 30 °C during 96 h of the fermentation period. It was found that the maximum yield of ethanol from sugar (0.44 g ethanol/g reducing sugar) was obtained with the substrate C/N ratio of 29.16 in Izmir medium. The main difference was the dominant carbon source available as a substrate. It was detected that glucose concentration was about 2.5 times higher in Izmir medium, whereas xylose concentration was about two times higher in Edirne medium. The different results obtained with rice hulls from different origins could depend on the type of paddy as well as different cultivation conditions. These findings provide a valuable indicator for identifying suitable agricultural waste materials to be used as substrates for bioethanol production.

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Abbreviations

2,3-BD:

2,3-Butanediol

DT:

Doubling time, in hour

EtOH:

Ethanol concentration, in gram per liter

HAc:

Acetic acid

HCap:

Caprionate

Hepta:

Heptanoic acid

HFor:

Formic acid

HLac:

Lactic acid

HPr:

Propionate

HSuc:

Succinic acid

IsoBut:

Isobutyrate

IsoVal:

Isovalate

LB:

Luria–Bertani medium

Q EtOH :

Volumetric productivity, in gram EtOH per liter per hour

X :

Biomass concentration, in gram per liter

VFAs:

Volatile fatty acids, in gram per liter

Y AB :

Yield of A from B, in gram per gram

μ :

Specific growth rate, in hour

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Acknowledgments

This study was a part of the COST Action FP0602, and the authors wish to thank The Scientific and Technical Research Council of Turkey (Tubitak) for the financial support.

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Authors and Affiliations

  1. Department of Bioengineering, Faculty of Engineering, University of Ege, 35100, Bornova, Izmir, Turkey

    Esra Imamoglu, Meltem Conk Dalay & Fazilet Vardar Sukan

Authors
  1. Esra Imamoglu
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  2. Meltem Conk Dalay
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  3. Fazilet Vardar Sukan
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Correspondence to Esra Imamoglu.

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Imamoglu, E., Dalay, M.C. & Sukan, F.V. Regional Differences in Rice Hulls Supply for Bioethanol Production. Appl Biochem Biotechnol 171, 2065–2074 (2013). https://doi.org/10.1007/s12010-013-0504-8

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